Abstract
Receptor for phage PIK specific for Pseudomonas aeruginosa strain PAO1 was studied. Phage PIK was strongly inactivated by lipopolysaccharide (LPS)_in vitro, exhibiting a PhI50 of 4.8 μg/ml. Further it was noted that this inactivation by LPS was reduced to 50% by several mono- and disaccharides when tested in vitro. d-glucosamine, d-mannose and l-rhamnose were found to be most effective at the concentration of 0.045 M, 0.25 M and 0.35 M respectively. This suggests the possibility that phage PIK receptor in LPS contains d-mannose, l-rhamnose and d-glucosamine. Either one of the former two could be located at a terminal position alpha-linked to the adjacent residue or located internally in the polysaccharide chain linked through its C-4 position. A theoretical approach to the interpretation of phage cell interaction was also investigated.
Abbreviations
- LPS:
-
Lipopolysaccharide
References
Bartell PF, Orr TE, Reese JF, Imaeda T (1971) Interaction of Pseudomonas bacteriophage 2 with slime polysaccharide and lipopolysaccharide of Pseudomonas aeruginosa strain BI. J Virol 8: 311–317
Bradley DE (1966) The structure and infective process of a Pseudomonas aeruginosa bacteriophage containing ribonucleic acid. J Gen Microbiol 45:83–96
Bradley DE, Pitt TL (1974) Pilus dependence of four Pseudomonas aeruginosa bacteriophage with non contractile tails. J Gen Virol 23:1–15
Castillo FJ, Bartell PF (1974) Studies on bacteriophage 2 receptor of Pseudomonas aeruginosa. J Virol 14:904–909
Huggest A St. G, Nixon DA (1957) Enzymatic determination of blood glucose. Biochem J 66:12P
Jacoby GA (1974) Properties of R-plasmids determining gentamycin resistance by acetylation in Pseudomonas aeruginosa. Antimicrob Ag Chemother 6:239–259
Jarrell K, Kropinski AMB (1976) The isolation characterization of a lipopolysaccharide specific Pseudomonas aeruginosa bacteriophage. J Gen Virol 33:99–106
Jarrell K, Kropinski AMB (1978) The chemical composition of the lipopolysaccharide from Pseudomonas aeruginosa strain PAO and a spontaneously derived rough mutant. Microbios 19:103–116
Kropinski AMB, Chan L, Jarrell K, Milazzo FH (1977) The nature of Pseudomonas aeruginosa Strain PAO bacteriophage receptors. Can J Microbiol 23:653–658
Lindberg AA (1967) Studies on a receptor for Feli 0–1 phage in Salmonella minnoesota. J Gen Microbiol 48:225–233
Lindberg AA (1973) Bacteriophage receptors. Ann Rev Microbiol 27:205–241
Meadow PM, Wells PL (1978) Receptor sites for R-pyocins and bacteriophage E-79 in the core part of the lipopolysaccharide of Pseudomonas aeruginosa PAO1. J Gen Microbiol 108:339–343
Osborne MJ (1963) Studies on Gram-negative cellwall. I. Evidence for the role of 2-keto 3-deoxyoctonate in the lipopolysaccharide of Salmonella typhimurium. Proc Natl Acad Sci USA 50:499–506
Rapin AMC, Kalckar HM (1971) The relation of bacteriophage attachment of lipopolysaccharide structure. In: Weinbaum G, Kadis S, Ajl SJ (eds) Microbial toxins, vol IV. Academic Press, New York London, pp 267–307
Robbins PW, Uchida T (1962) Studies on the chemical basis of the phage conversion of O-antigen in the E group Salmonellae. Biochemistry 1:323–335
Schlesinger M (1932) Adsorption of bacteriophages to homologous bacteria. II. Quantitative investigation of adsorption velocity and saturation; estimation of the particle size of the bacteriophage. Z Hyg Immunitaetsforsch 114:149–160 (Reprinted in Translation 1960) Stent GS (ed) Papers on bacterial viruses. Little, Brown and Co., Boston, pp 26–36
Weidel W (1958) Bacterial viruses (with particular reference to adsorption/penetration). Ann Rev Microbiol 12:27–48
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Patel, I.R., Rao, K.K. Studies on the Pseudomonas aeruginosa PAO1 bacteriophage receptors. Arch. Microbiol. 135, 155–157 (1983). https://doi.org/10.1007/BF00408026
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DOI: https://doi.org/10.1007/BF00408026