Abstract
Lipopolysaccharides were isolated from two strains of Thiobacillus ferrooxidans and one strain each of Thiobacillus thiooxidans, Thiobacillus novellus and Thiobacillus sp. IFO 14570. Neutral sugars, 2-keto-3-deoxyoctonate, fatty acids and the rare 2,3-diamino-2,3-dideoxyglucose were detected in all lipopolysaccharides. Lipopolysaccharides of both T. ferrooxidans strains contained l-glycero-d-manno-heptose, whereas that of T. thiooxidans contained both l-glycero-d-manno-heptose and d-glycero-d-manno-heptose. On the other hand, heptoses were absent in lipopolysaccharides of T. novellus and Thiobacillus sp. IFO 14570. Lipid A of T. ferrooxidans and T. thiooxidans contained both glucosamine and 2,3-diamino-2,3-dideoxyglucose, in contrast, lipid A of T. novellus and the Thiobacillus sp. IFO 14570 most likely contain only 2,3-diamino-2,3-dideoxyglucose as backbone sugar. Deoxycholate polyacrylamide gel electrophoresis revealed S-type character for all lipopolysaccharides studied. The significance of the lipopolysaccharide composition for taxonomic and phylogenetic questions with regard to thiobacilli is discussed.
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Abbreviations
- DAG:
-
2,3-diamino-2,3-dideoxyglucose
- DOC:
-
sodium deoxycholate
- GC:
-
gas-liquid chromatography
- GC/MS:
-
gas-liquid chromatography/mass spectrometry
- d,d-Heptose:
-
d-glycero-d-manno-heptose
- l,d-Heptose:
-
l-glycero-d-manno-heptose
- KDO:
-
2-keto-3-deoxyoctonate
- LPS:
-
lipopolysaccharide
- 3-OH-14:0:
-
3-hydroxy-tetradecanoic acid
- PAGE:
-
polyacrylamide gel electrophoresis
- PCP:
-
phenol-chloroform-petroleum ether
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Yokota, A., Rodriguez, M., Yamada, Y. et al. Lipopolysaccharides of Thiobacillus species containing lipid A with 2,3-diamino-2,3-dideoxyglucose. Arch. Microbiol. 149, 106–111 (1987). https://doi.org/10.1007/BF00425074
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DOI: https://doi.org/10.1007/BF00425074