Abstract
Diamino butanone (DAB), a competitive inhibitor of ornithine decarboxylase (ODC) a key enzyme in polyamine biosynthesis, inhibited the yeast to hyphae transition in Mucor rouxii, induced by transfer from anaerobiosis to aerobiosis, but not the opposite phenomenon. Addition of DAB to anaerobic yeast cells brought about a decrease in ODC and polyamine levels. In these conditions, the aerobic shift produced only a weak increase in ODC activity and no change in polyamine levels. DAB also blocked phorogenesis in M. rouxii and in Phycomyces blakesleeanus. At the effective concentrations DAB did not affect cell growth of either fungus. It is suggested that low, constant levels of ODC and polyamines are necessary for cell growth, and that high transient levels are required during the differentiative steps. DAB, at the concentrations used, affects this last process, but does not interfere with the maintenance level of polyamines.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- ODC:
-
ornithine decarboxylase
- DAB:
-
1,4-diamino butanone
References
Bartnicki-Garcia S (1963) Mold-yeast dimorphism of Mucor. Bacteriol Rev 27:293–304
Bartnicki-Garcia S, Nickerson WJ (1962) Nutrition, growth and morphogenesis of Mucor rouxii. J Bacteriol 84:841–858
Basabe JR, Lee CA, Weiss RL (1979) Enzyme assay using permeabilized cells of Neurospora. Anal Biochem 92:356–360
Calvo-Mendez C, Martinez-Pacheco M, Ruiz-Herrera J (1987) Regulation of ornithine decarboxylase activity in Mucor bacilliformis and Mucor rouxii. Exp Mycol 11:270–277
Garcia JR, Hiatt WR, Peters J, Sypherd PS (1980) S-Adenosylmethionine levels and protein methylation during morphogenesis of Mucor racemosus. J Bacteriol 142:196–201
Heby O (1981) Role of polyamines in the control of cell proliferation and differentiation. Differentiation 19:1–20
Inderlied CB, Cihlar RL, Sypherd PS (1980) Regulation of ornithine decarboxylase during morphogenesis of Mucor racemosus. J Bacteriol 141:696–706
Inderlied CB, Peters J, Cihlar RL (1985) Mucor racemosus. In: Szaniszlo PJ (ed) Fungal dimorphism. Plenum Press, New York, pp 337
Kim WK (1971) Folate and polyamine of undifferentiated and differentiated wheat stem rust uredosporelings. Can J Biol 49:1119–1122
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Menucci L, Rojas S, Camargo EP (1975) Polyamines and ornithine decarboxylase activity during growth and differentiation in Blastocladiella emersonii. Biochim Biophys Acta 404:109–123
Paulus TJ, Davis RH (1981) Regulation of polyamine synthesis in relation to putrescine and spermidine pools in Neurospora crassa. J Bacteriol 145:14–20
Paulus TJ, Davis RH (1982) Metabolic sequestration of putrescine in Neurospora crassa. Biochem Biophys Res Commun 104:228–233
Ruiz-Herrera J, Calvo-Mendez C (1987) Effect of ornithine decarboxylase inhibitors on the germination of sporangiospores of Mucorales. Exp Mycol 11:287–296
Russell DH (1981) Posttranslational modification of ornithine decarboxylase by its product putrescine. Biochem Biophys Res Commun 99:1167–1172
Russell DH, Snyder SH (1968) Amine synthesis in rapidly growing tissues: ornithine decarboxylase activity in regenerating rat liver, chick embryos and various tumors. Proc Natl Acad Sci USA 66:1420–1427
Seiler N (1983) Liquid chromatographic methods for assaying polymines using prechromatographic derivatization. Meth Enzymol 94:10–25
Tabor CW, Tabor H (1976) 1,4-Diaminobutane (putrescine), spermidine and spermine. Ann Rev Biochem 45:285–306
Tabor CW, Tabor H (1984) Polyamines. Ann Rev Biochem 53:749–790
Tabor CW, Tabor H (1985) Polyamines in microorganisms. Microbiol Rev 49:81–99
Tabor CW, Tabor H, Tyagi AK, Cohn MS (1982) The biochemistry, genetics and regulation of polyamine biosynthesis in Saccharomyces cerevisiae. Fed Proc 41:3084–3088
Winther M, Stevens L (1978) Polyamine stimulation of in vivo rates of macromolecular synthesis in a putrescine auxotroph of Aspergillus nidulans. FEBS Lett 85:229–232
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Martinez-Pacheco, M., Rodriguez, G., Reyna, G. et al. Inhibition of the yeast-mycelial transition and the phorogenesis of Mucorales by diamino butanone. Arch. Microbiol. 151, 10–14 (1988). https://doi.org/10.1007/BF00444661
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00444661