Skip to main content
Log in

Effect of iron deficiency on ferredoxin levels in Anabaena variabilis PCC 6309

  • Original Papers
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

Cultures of the nitrogen-fixing cyanobacterium Anabaena variabilis PCC 6309 were grown under several iron concentrations, and changes in growth rates and chlorophyll and phycocyanin concentrations were determined. The total amount of ferredoxin present in the cells was found to be dependent on the concentration of iron in the media, as was the pattern distribution of the two different forms of the iron-sulfur protein described in this organism. Flavodoxin was not found to be present in these cells even when they were grown in the absence of added iron, indicating that this flavoprotein does not replace ferredoxin in this particular strain.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • AllenMM, SmithAJ (1969) Nitrogen chlorosis in blue-green algae. Arch Microbiol 69: 114–120

    Google Scholar 

  • BuchananB, ArnonDI (1971) Ferredoxins from photosynthetic bacteria, algae and higher plants. Methods Enzymol 23: 413–441

    Google Scholar 

  • CammackR, RaoKK, BargeronCP, HutsonKG, AndrewPW, RogersLJ (1977) Midpoint redox potentials of plant and algal ferredoxins. Biochem J 168: 205–209

    Google Scholar 

  • ChanTM, MarkleyJL (1983) Nuclear magnetic resonance studies of two-iron-two-sulfur ferredoxins. 1. Properties of the histidine residues. Biochemistry 22: 5982–5987

    Google Scholar 

  • FillatMF, SandmannG, Gómez-MorenoC (1988) Flavodoxin from the nitrogen-fixing cyanobacterium Anabaena PCC 7119. Arch Microbiol 150: 160–164

    Google Scholar 

  • GlazerAN (1976) Phycocyanins: structure and function. Photochem Photobiol Rev 1: 71–115

    Google Scholar 

  • GuikemaJA, ShermanLA (1983) Organization and function of chlorophyll in membranes of cyanobacteria during iron starvation. Plant Physiol 73: 250–256

    Google Scholar 

  • GuikemaJA, ShermanLA (1984) Influence of iron deprivation on the membrane composition of Anacystis nidulans. Plant Physiol 74: 90–95

    Google Scholar 

  • HoKK, KrogmanDW (1982) Photosynthesis. In: CarrNG, WhittonBA (eds) The biology of cyanobacteria. Blackwell, London, pp 191–214

    Google Scholar 

  • ManzanoC, CandauP, Gómez-MorenoC, RelimpioAM, LosadaM (1976) Ferredoxin dependent photosynthetic reduction of nitrate and nitrite by particules of Anacystis nidulans. Mol Cell Biochem 10: 161–169

    Google Scholar 

  • PakrasiHB, GoldenbergA, ShermanLA (1969) Membrane development in the cyanobacterium Anacystis nidulans during recovery from iron starvation. Plant Physiol 79: 290–295

    Google Scholar 

  • ParsonsHB, StricklandTR (1973) Pigment analysis. In: SteinJR (ed) Handbook of phycological methods: culture methods and growth measurements. Cambridge University Press, Cambridge, pp 360–367

    Google Scholar 

  • RippkaR, DeruellesJ, WaterburyJB, HermanM, StanierRY (1979) Generic assignements, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111: 1–61

    Google Scholar 

  • RogersLJ (1987) Ferredoxins, flavodoxins and related proteins: structure, function and evolution. In: VanBaalenP, FayC (eds) The cyanobacteria. Elsevier, Amsterdam, pp 35–67

    Google Scholar 

  • SandmannG, BögerP (1983) The enzymological function of heavy metals and their role in electron transfer processes of plants. In: LanchliA, BileskiRK (eds). Inorganic plant nutrition. Encyclopedia of plant physiology vol 12. Springer, Berlin Heidelberg New York, pp 563–596

    Google Scholar 

  • WadaK, MatsubaraH, ChainRK, ArnonDI (1981) A comparative study of the biological activities of two molecular species of chloroplast-type ferredoxins. Plant Cell Physiol 22: 275–281

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pardo, M.B., Gómez-Moreno, C. & Peleato, M.L. Effect of iron deficiency on ferredoxin levels in Anabaena variabilis PCC 6309. Arch. Microbiol. 153, 528–530 (1990). https://doi.org/10.1007/BF00245260

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00245260

Key words

Navigation