Skip to main content
SpringerLink
Log in

Changes in the cell wall glycoprotein composition of Candida albicans associated to the inhibition of germ tube formation by EDTA

  • Original Papers
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

Hyphal development in Candida albicans was blocked by EDTA. This effect was not due to a general growth inhibition since the chelator did not affect protein and DNA synthesis. Recovery of mycelial growth was observed when EDTA-grown cells were incubated at 37°C in EDTA-free medium. High-molecular-weight mannoproteins (HMWM) that are mycelium-specific wall components, and particularly a 260-kDa species (HMWM-260), were absent in the wall of cells grown under germination conditions in the presence of EDTA. Synthesis of the HMWM-260 species was not inhibited but its incorporation (secretion) into the wall structure seemed to be blocked in EDTA-treated cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anderson JM, Soll DR (1986) Differences in actin localization during bud and hypha formation in the yeast Candida albicans. J Gen Microbiol 132: 2035–2047

    Google Scholar 

  • Calderone RA, Braun PC (1991) Adherence and receptor relationships in Candida albicans. Microbiol Rev 55: 1–20

    Google Scholar 

  • Casanova M, Gil ML, Cardeñoso L, Martínez JP, Sentandreu R (1989) Identification of wall-specific antigens synthesized during germ tube formation by Candida albicans. Infect Immun 57: 262–271

    Google Scholar 

  • Casanova M, Martínez JP, Chaffin WL (1990) Fab fragments from a monoclonal antibody against germ tube specific mannoprotein block yeast-to-mycelium transition in Candida albicans. Infect Immun 50: 3810–3812

    Google Scholar 

  • Casanova M, López-Ribot JL, Martínez JP, Sentandreu R (1992) Characterization of cell wall proteins from yeast and mycelial cells of Candida albicans by labelling with biotin. Comparison with other techniques. Infect Immun 60:4898–4906

    Google Scholar 

  • Cassone A (1989) Cell wall of Candida albicans: its functions and its impact on the host. In: McGinnis MR, Borgers M (eds) Current topics in medical mycology, vol 3. Springer, New York, pp 53–70

    Google Scholar 

  • Chaffin WL (1985) The effect on tunicamycin on germ tube and yeast bud formation in Candida albicans. J Gen Microbiol 131:1853–1861

    Google Scholar 

  • Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356

    Google Scholar 

  • Elorza MV, Rico H, Gozalbo D, Sentandreu R (1983) Cell wall composition and protoplast regeneration in Candida albicans. Antonie Van Leeuwenhoek J Microbiol Serol 49:457–469

    Google Scholar 

  • Elorza MV, Murgui A, Sentandreu R (1985) Dimorphism in Candida albicans: contribution of mannoprotein to the architecture of yeast and mycelial cell walls. J Gen Microbiol 131:2209–2216

    Google Scholar 

  • Elorza MV, Murgui A, Rico A, Miragall F, Sentandreu R (1987) Formation of a new cell wall by protoplasts of Candida albicans: effect of papulacandin B, tunicamycin and nikkomycin. J Gen Microbiol 133:2315–2325

    Google Scholar 

  • Elorza MV, Mormeneo S, Garcia de la Cruz F, Gimeno C, Sentandreu R (1989) Evidence for the formation of covalent bonds between macromolecules in the domain of the wall of Candida albicans mycelial cells. Biochem Biophys Res Commun 162:1118–1125

    Google Scholar 

  • Fukuyama M, Calderone RA (1991) Adherence of cell surface mutants of Candida albicans to buccal epithelial cells and analyses of the cell surface proteins of the mutants. Infect Immun 59:1341–1345

    Google Scholar 

  • Gil ML, Casanova M, Martínez JP, Sentandreu R (1991) Antigenic cell wall mannoproteins in Candida albicans isolates and in other Candida species. J Gen Microbiol 137:1053–1061

    Google Scholar 

  • Gupta Roy B, Datta A (1987) A calmodulin inhibitor blocks morphogenesis in Candida albicans. FEMS Microbiol Lett 41:327–329

    Google Scholar 

  • Klee CB, Crouch TH, Richman PG (1980) Calmodulin. Annu Rev Biochem 49:489–515

    Google Scholar 

  • Lee KL, Buckley MR, Campbell C (1975) An amino acid liquid synthetic medium for development of mycelial and yeast forms of Candida albicans. Sabouraudia 13:148–153

    Google Scholar 

  • Leemann U, Ruch F (1982) Cytofluorimetric determination of DNA base content in plant nuclei and chromosomes by the fluorochrome DAPI and cromomycin A3. Exp Cell Res 140:275–282

    Google Scholar 

  • López-Ribot JL, Casanova M, Martínez JP, Sentandreu R (1991) Characterization of cell wall proteins of yeast and hydrophobic mycelial cells of Candida albicans. Infect Immun 59:2324–2332

    Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    Google Scholar 

  • Lydan MA, O'Day DH (1988) Differential developmental functions for calmodulin in Dictyostelium: trifluoperazine and R24571 both inhibit cell and pronuclear fusion but enhance gamete formation. Exp Cell Res 178:51–63

    Google Scholar 

  • Martínez JP, Gil ML, Casanova M, López-Ribot JL, García de Lomas J, Sentandreu R (1990a) Wall mannoproteins in cells from colonial phenotypic variants of Candida albicans. J Gen Microbiol 136:2421–2432

    Google Scholar 

  • Martínez JP, López-Ribot JL, Gil ML, Sentandreu R, Ruiz-Herrera J (1990b) Inhibition of the dimorphic transition of Candida albicans by the ornithine decarboxylase inhibitor 1,4-diaminobutanone: alterations in the glycoproteins composition of the cell wall. J Gen Microbiol 136:1937–1943

    Google Scholar 

  • Means AR, Dedman JR (1980) Calmodulin — an intracellular calcium receptor. Nature 285:73–77

    Google Scholar 

  • Molinari A, Gomez MJ, Crateri P, Torosantucci A, Cassone A, Arancia G (1993) Differential cell surface expression of mannoprotein epitopes in yeast and mycelial forms of Candida albicans. Eur J Cell Biol 60:146–153

    Google Scholar 

  • Murgui A, Elorza MV, Sentandreu R (1985a) Effect of papulacandin B and calcofluor white on the incorporations of mannoproteins in the cell wall of Candida albicans blastospores. Biochim Biophys Acta 841:215–222

    Google Scholar 

  • Murgui A, Elorza MV, Sentandreu R (1985b) Tunicamycin and papulacandin B inhibit incroporation of specific mannoproteins into the wall of Candida albicans regenerating protoplasts. Biochim Biophys Acta 884:550–558

    Google Scholar 

  • Muthukumar G, Nickerson KW (1984) Ca(II)-calmodulin regulation of fungal dimorphism in Ceratocystis ulmi. J Bacteriol 159:390–392

    Google Scholar 

  • Muthukumar G, Nickerson KW (1985) Ca(II)-calmodulin regulation of morphological commitment in Ceratocystis ulmi. FEMS Microbiol Lett 27:199–202

    Google Scholar 

  • Odds FC (1988) Candida and candidosis. A review and bibliography. Bailliere Tindall, London

    Google Scholar 

  • Paranjape V, Gupta Roy B, Datta A (1990) Involvement of calcium, calmodulin and protein phosophorylation in morphogenesis of Candida albicans. J Gen Microbiol 136:2149–2154

    Google Scholar 

  • Pringle JR, Lillie SH, Adams AEM, Jacobs CW, Haarer BK, Coleman KG, Robinson JS, Bloom L, Preston RA (1986) Cellular morphogenesis in the yeast cell cycle. In: Yeast cell biology. Alan R Liss, New York, pp 47–80

    Google Scholar 

  • Sabie FT, Gadd GM (1989) Involvement of a Ca2+-calmodulin interaction in the yeast-mycelial (Y-M) transition of Candida albicans. Mycopathologia 108:47–54

    Google Scholar 

  • Sentandreu R, Martínez JP, Elorza MV, Mormeneo S (1991) Relationships between dimorphism, cell wall structure and surface activities in Candida albicans. In: Prasad R (ed) Candida albicans: cellular and molecular biology. Springer, New York, pp 53–70

    Google Scholar 

  • Slutsky B, Buffo B, Soll DR (1985) High-frequency “switching” of colony morphology in Candida albicans. Science 230:666–669

    Google Scholar 

  • Slutsky B, Staebell M, Anderson J, Risen L, Pfaller M, Soll DR (1987) “White-Opaque transition”: a second high-frequency switching system in Candida albicans. J Bacteriol 169:189–197

    Google Scholar 

  • St Leger R, Roberts DW, Staples RC (1989) Calcium and calmodulin-mediated protein synthesis and protein phosphorylation during germination, growth and protease production by Metarhizium anisopliae. J Gen Microbiol 135:2141–2154

    Google Scholar 

  • Uyeda TQ, Furya M (1986) Effect of low temperature and calcium on microfilament structure in flagellates of Physarum polycephalum. Exp Cell Res 165:461–472

    Google Scholar 

  • Wessels JGH (1986) Cell wall synthesis in apical hyphal growth. Int Rev Cytol 104:37–79

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Sección Departamental de Microbiología, Facultad de Farmacia, Universitat de Valencia, Avda. Vicent Andrés Estellés, s/n, E-46100-Burjassot, Valencia, Spain

    M. Luisa Gil, Manuel Casanova & José P. Martínez

Authors
  1. M. Luisa Gil
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Manuel Casanova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. José P. Martínez
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gil, M.L., Casanova, M. & Martínez, J.P. Changes in the cell wall glycoprotein composition of Candida albicans associated to the inhibition of germ tube formation by EDTA. Arch. Microbiol. 161, 489–494 (1994). https://doi.org/10.1007/BF00307769

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00307769

Key words

Search

Navigation