Abstract
The therapeutic effect of Vitamin B12 or Vitamin B6 on 2,5-hexanedione induced-neuropathy in animals was examined by using our electrodiagnostic technique in order to investigate the mechanism of the development of the neuropathy.
Pyridoxal phosphate and two forms of Vitamin B12 were administered to rats intoxicated by the neurotoxin for a period of 18 weeks, and the sensory and motor fiber conduction velocity and the motor distal latency of the tail nerve were periodically determined. None of the groups treated with the therapeutic drugs exhibited a definite improvement in the nerve fiber conduction velocity and motor distal latency of the rat tail nerve, as compared with those of the 2,5-hexane-dione-treated controls.
This is a preview of subscription content, log in via an institution to check access.
References
Biel JP, Vilter RE (1954a) Effects of isoniazid on pyridoxine metabolism. JAMA 156: 1549–1552
Biel JP, Vilter RW (1954b) Effect of isoniazid on vitamin B6 metabolism; Its possible significance in producing isoniazid neuritis. Proc Soc Exp Biol Med 85: 389–392
Committee of International Nutrition Programs (1972) Vitamin B6 deficiency and brain lipids in the suckling rat. Nutr Rev 30: 183–186
Committee of International Nutrition Programs (1978) Vegetarian diet and Vitamin B12 deficiency. Nutr Rev 36: 243–244
Decaprio AP, Olajos EJ, Weber P (1982) Covalent binding of a neurotoxic n-hexane metabolite: Conversion of primary amines to substituted pyrrole adducts by 2,5-hexanedione. Toxicol Appl Pharmacol 65: 440–450
Dyck PJ, Tohmas PK, Lambert EH (1975a) Peripheral neuropathy, vol I. WB Sanders Co., Philadelphia p323
Dyck PJ, Tohmas PK, Lambert EH (1975b) Peripheral neuropathy, vol II. WB Sanders Co., Philadelphia p 1053
Gillis PJ, Norton RM, Bus JS (1980) Effect of 2,5-hexanedione on lipid biosynthesis in sciatic nerve and brain of the rat. Toxicol Appl Pharmacol 54: 210–216
Graham DG, Anthony DC, Boekelheide K, Mascmann NA, Richards RG, Wolfram JW, Shaw BR (1982) Studies of the molecular pathogenesis of hexane neuropathy. II Evicence that pyrrole derivazation of lysyl residues leads to protein cross linking. Toxicol Appl Pharmacol 64: 415–422
Mayer RF (1965) Peripheral nerve function in vitamin B12 deficiency. Arch Neurol 13: 355–362
Misumi J (1979) Electrophysiological studies in vivo on peripheral nerve function and their application to peripheral neuropathy produced by organic mercury in rats. II Measurement of the maximum conduction velocity of compound action potentials in the tail nerve of healthy rats. Kumamoto Med J 32: 1–14
Misumi J, Kawakami M, Nomura S (1979) Effects of n-hexane, methyl n-butyl ketone and 2,5-hexanedione on the conduction velocity of motor and sensory fibers in the rats tail. Jpn J Ind Med 21: 180–181
Nagano M, Misumi J, Nomura S (1983) An electrophysiological study on the peripheral neurotoxicity of 2,3-butanedione, 2,4-pentanedione and 2,5-hexanedione in rats. Jpn J Ind Med 25: 471–482
Ohnishi A, Kuroiwa Y, Ohgo T (1979) The effect of methylcobalamin on the experimental acrylamide neuropathy-Morphometric study. Clin Pharmacol 10: 247–251
Sabri MI, Moore CL, Spencer PS (1979) Studies on the biochemical basis of distal axonopathies I. Inhibition of glycolysis by neurotoxic hexacarbon compounds. J Neurochem 32: 683–689
Yagihashi S, Nishihira M, Kashiwamura H, Tokui A (1980) Quantitative analysis of the effect of methylcobalamin on the peripheral nerve structures in experimental diabetic neuropathy. Clin Pharmacol 11: 131–139
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Misumi, J., Nagano, M., Kaisaku, J. et al. Effects of vitamin B12 and B6 on 2,5-hexanedione-induced neuropathy. Arch Toxicol 56, 204–206 (1985). https://doi.org/10.1007/BF00333428
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00333428