Abstract
A defined mixture of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDDs and PCDFs) was parenterally administered to rats and absorption and tissue distribution were measured: 1) Toluene/DMSO (1+2; v/v) proved to be a convenient vehicle for the subcutaneous administration of the various PCDDs and PCDFs. Seven days after application the rate of absorption was 90% of the administered dose or even higher for almost all of the PCDDs/PCDFs in the mixture. In a few cases only (e.g. OCDD) the rate was found to be 84–89%; 2) Seven days after subcutaneous administration all 2378-substituted congeners were found in the liver, whereas only a few non-2378-substituted congeners could be measured in minor quantities. The 2378-substituted congeners also predominated in adipose tissue; however, most of the non-2378-substituted congeners were also detected; 3) The amount deposited within the liver as percentage of the administered dose differed for the various 2378-substituted PCDDs and PCDFs, ranging from <10% for OCDD or 2378-T4CDF, and between 60 and close to 100% for 12378-P5CDD or the H6CDDs. Therefore, the concentration ratio (liver/adipose tissue) was also found to be very different, ranging from <3 in the case of 2378-T4CDD or 2378-T4CDF to >40 in the case of 1234678-H7CDD, 23478-P5CDF, 123678-H6CDF, or 1234678-H7CDF; 4) Studies performed at the time period of ongoing absorption (13–14 h after injection) provided the first evidence that some of the non-2378-substituted congeners do reach substantial concentrations in hepatic tissue shortly after administration; 5) Subsequent to intraperitoneàl injection of the same PCDD/PCDF mixture the concentrations within the liver were found to be almost identical with that found after subcutaneous injection. In contrast, much higher concentrations of the congeners were found in (abdominal) adipose tissue; 6) In the liver of untreated rats of the same strain no T4CDDs/T4CDFs
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- PCDDs, PCDFs:
-
polychlorinated dibenzo-p-dioxins, and dibenzofurans
- T4CDDs, T4CDFs:
-
tetra-chlorinated dibenzo-p-dioxins, and dibenzofurans
- P5CDDs, P5CDFs:
-
penta-chlorinated dibenzo-p-dioxins, and dibenzofurans
- H6CDDs, H6CDFs:
-
hexa-chlorinated dibenzo-p-dioxins, and dibenzofurans
- H7CDDs, H7CDFs:
-
hepta-chlorinated dibenzo-p-dioxins, and dibenzofurans
- OCDD, OCDF:
-
octa-chlorinated dibenzo-p-dioxin, and dibenzofuran
- DMSO:
-
dimethylsulfoxide
References
Abraham K, Krowke R, Neubert D (1988) Pharmacokinetics and biological activity of 2,3,7,8-tetrachlorodibenzo-p-dioxin: 1. Dose-dependent tissue distribution and induction of hepatic ethoxyresorufin O-deethylase in rats following a single injection. Arch Toxicol 62: 359–368
Abraham K, Krowke R, Neubert D (1989a) Absorption of TCDD following parenteral application in rats using various vehicles. Chemosphere (in press)
Abraham K, Wiesmüller T, Brunner H, Krowke R, Hagenmaier H, Neubert D (1989b) Elimination of various polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDDs and PCDFs) in rat faeces. Arch Toxicol (in press)
Beck H, Eckart K, Mathar W, Wittkowski R (1988) Levels of PCDDs and PCDFs in adipose tissue of occupationally exposed workers. Chemosphere (in press)
Beck H, Eckart K, Mathar W, Wittkowski R (1987) Isomerenspezifische Bestimmung von PCDD und PCDF in Human- und Lebensmittelproben. VDI Berichte 634: 359–382
Brunner H, Wiesmüller T, Hagenmaier H, Abraham K, Krowke R, Neubert D (1989) Distribution of PCDDs and PCDFs in rat tissues following various routes of administration. Chemosphere (in press)
Decad GM, Birnbaum LS, Matthews HB (1982) Disposition of 2,3,7,8-tetrachlorodibenzofuran in Guinea pigs, rats and monkeys. In: Hutzinger O, Frei RW, Merian E, Pocchiari F (eds) Chlorinated dioxins and related compounds. Pergamon Press, Oxford New York Toronto, pp 307–315
Hagenmaier H, Brunner H, Haag R, Kraft M (1987) Copper-catalyzed dechlorination/hydrogenation of polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans, and other chlorinated aromatic compounds. Environ Sci Technol 21: 1085–1088
Hagenmaier H, Brunner H, Wiesmüller T, Haag R, Abraham K, Krowke R, Neubert D (1989) Preparation of defined mixtures of PCDDs and PCDFs for studying toxicokinetic and toxicological properties. Chemosphere (in press)
Kuroki H, Masuda Y, Yoshihara S, Yoshimura H (1980) Accumulation of polychlorinated dibenzofurans in the livers of monkeys and rats. Fd Cosmet Toxicol 18: 387–392
Poiger H, Pluess N, Schlatter C (1988) Subchronic toxicity of some chlorinated dibenzofurans in rats. Chemosphere (in press)
Rappe C, Nygren M, Lindström G, Hansson M (1986) Dioxins and dibenzofurans in biological samples of European origin. Chemosphere 15: 1635–1639
Rose JQ, Ramsey JC, Wentzler TH, Hummel RA, Gehring PJ (1976) The fate of 2,3,7,8-tetrachlorodibenzo-p-dioxin following single and repeated oral doses to the rat. Toxicol Appl Pharmacol 36: 209–226
Ryan JJ (1986) Variation of dioxins and furans in human tissues. Chemosphere 15: 1585–1593
Schecter AJ, Ryan JJ, Constable JD (1986) Chlorinated dibenzo-p-dioxin and dibenzofuran levels in human adipose tissue and milk samples from the north and south of Vietnam. Chemosphere 15: 1613–1620
Schecter A, Ryan JJ, Lizotte R, Sun WF, Miller L, Gitlitz G, Bogdasarian M (1985) Chlorinated dibenzodioxins and dibenzofurans in human adipose tissue from exposed and control New York State patients. Chemosphere 14: 933–937
Schlatter C (1987) Risiko-Abschätzung (Toxizitätsäquivalente). VDI Berichte 634: 503–514
UBA, Umweltbundesamt (1985) Sachstand Dioxine. Berichte 5/85. Erich Schmidt Verlag, Berlin, p 264
Van den Berg M, Heeremans C, Meerman L, Veenhoven E, van Wijnen J, Olie K, Hutzinger O (1986) Some pharmacokinetic aspects of PCDDs and PCDFs in mammals after administration of a fly ash extract from a municipal incinerator. Chemosphere 15: 1477–1487
Wacker R, Poiger H, Schlatter C (1986) Pharmacokinetics and metabolism of 1,2,3,7,8-pentachlorodibenzo-p-dioxin in the rat. Chemosphere 15: 1473–1476
Williams DT, Cunningham HM, Blanchfield BJ (1972) Distribution and excretion studies of octachlorodibenzo-p-dioxin in the rat. Bull Environ Contam Toxicol 7: 57–62
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Abraham, K., Wiesmüller, T., Brunner, H. et al. Absorption and tissue distribution of various polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDDs and PCDFs) in the rat. Arch Toxicol 63, 193–202 (1989). https://doi.org/10.1007/BF00316368
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00316368