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Über Lipide, insbesondere Lysolecithin, in den chromaffinen Granula verschiedener Species

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Summary

1. Chromaffin granules and mitochondria were obtained from adrenal medullae of ox, horse, pig and rat and their lipid composition was investigated.

2. The major components of the lipids of each particle were phospholipids and cholesterol. Chromaffin granules were relatively rich in cholesterol.

3. In both particles the following phospholipids were detected by thin-layer chromatography: lysolecithin, lecithin, phosphatidylethanolamine (partly plasmalogen), phosphatidylserine, phosphatidylinostiol and sphingomyelin. Quantitative analysis revealed that chromaffin granules from ox, horse, pig and rat contained a high concentration of lysolecithin (7–17% of total phospholipids).

4. When the adrenal glands of rats were maximally stimulated with insulin, the phospholipid composition of the chromaffin granules did not change.

5. The possible role of lysolecithin in the secretion of catecholamines is discussed.

Zusammenfassung

1. Chromaffine Granula und Mitochondrien des Nebennierenmarks wurden durch Zellfraktionierung isoliert und ihre Lipide untersucht.

2. Chromaffine Granula und Mitochondrien von Rind, Pferd und Schwein enthalten als Lipid-Hauptkomponenten Phosphatide und Cholesterin, wobei Granula eine relativ höhere Konzentration von Cholesterin als Mitochondrien enthalten.

3. Als Phosphatidfraktionen konnten in den chromaffinen Granula und Mitochondrien von Rind, Pferd, Schwein und Ratte Lecithin, Lysolecithin, Colamin-Kephalin, Serin-Kephalin, Inositphosphatid und Sphingomyelin festgestellt und quantitativ bestimmt werden. Bemerkenswert war der hohe Gehalt chromaffiner Granula an Lysolecithin (7–17% der Gesamtphosphatide).

4. Die Phosphatidzusammensetzung von Rattengranula war nach intensiver Stimulierung der Adrenalinsekretion durch Insulingabe nicht verändert.

5. Die mögliche Bedeutung der hohen LL-Konzentrationen in chromaffinen Granula für den Sekretionsmechanismus der Katecholamine wird diskutiert.

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Authors and Affiliations

  1. Pharmakologisches Institut der Universität Innsbruck, Austria

    H. Winkler, N. Strieder & E. Ziegler

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  1. H. Winkler
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  2. N. Strieder
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  3. E. Ziegler
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Additional information

Über einen Teil dieser Ergebnisse wurde bereits auf der 7. Frühjahrstagung der Deutschen Pharmakologischen Gesellschaft in Mainz (1966) berichtet. [Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 255, 94 (1966).]

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Winkler, H., Strieder, N. & Ziegler, E. Über Lipide, insbesondere Lysolecithin, in den chromaffinen Granula verschiedener Species. Naunyn-Schmiedebergs Arch. Pharmak. u. Exp. Path. 256, 407–415 (1967). https://doi.org/10.1007/BF00536799

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  • DOI: https://doi.org/10.1007/BF00536799

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