Summary
The uptake of trace amounts of 3H-demethylphalloin (3H-DMP) by isolated hepatocytes was studied in the presence of various concentrations of unlabeled demethylphalloin (DMP), of phalloin and of phalloidin. The addition of phalloidin (or phalloin) reduces the uptake of 3H-DMP more than the addition of the equivalent concentration of DMP. The error caused by dilution of 3H-DMP with phalloin or phalloidin is not constant and depends on the concentration of the unlabeled compound. The relative differences between the uptake of 3H-DMP in the presence of demethylphalloin and in the presence of either phalloin or phalloidin cannot be explained by a competitive model. Some consequences for the use of 3H-DMP in toxicokinetic experiments are discussed.
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This work was supported by the Deutsche Forschungsgemeinschaft
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Frimmer, M., Petzinger, E., Ziegler, K. et al. Uptake of 3H-demethylphalloin by isolated hepatocytes in the presence of various concentrations of phalloin or phalloidin. Naunyn-Schmiedeberg's Arch. Pharmacol. 311, 91–94 (1980). https://doi.org/10.1007/BF00500308
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DOI: https://doi.org/10.1007/BF00500308