Summary
The release of dopamine, noradrenaline, adrenaline, histamine and GABA was studied in the posterior hypothalamus of conscious, freely moving Wistar Kyoto (WKy) rats and spontaneously hypertensive (SH) rats (Okamoto strain). A guide cannula was stereotaxically inserted under anaesthesia. Some days later a push-pull cannula was inserted into the guide cannula so that the tip of the push-pull cannula reached the posterior hypothalamic nucleus. The posterior hypothalamic nucleus of the freely moving animal was superfused with artificial CSF. The rates of release of the neurotransmitters were determined in the superfusate which was continuously collected in 10 min periods.
In SH rats, the rates of resting release of dopamine and histamine were higher than in WKy rats, while the rates of release of noradrenaline and adrenaline in SH rats were lower than those in WKy rats. No significant differences were found in the rates of resting release of GABA between SH and WKy rats. Superfusion of the posterior hypothalamic nucleus of WKy rats with KCl-rich CSF (60 mmol/1 KCl) significantly increased the rates of release of noradrenaline and adrenaline, while those of dopamine and GABA tended to be enhanced. In SH rats, hypothalamic superfusion with KCl-rich CSF increased the rates of release of dopamine, noradrenaline, adrenaline and GABA. The KCl-induced release of neurotransmitters did not differ between SH and WKy rats. Superfusion with KCl-rich CSF did not influence the rates of release of histamine either in SH or in WKy rats.
The findings indicate that differences exist in the resting release of dopamine, noradrenaline, adrenaline and histamine between SH and WKy rats. Our experimental set-up seems to be useful in investigating neurochemical changes in distinct brain areas of animals at different physiopathologic stages under in vivo conditions.
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This work was supported by the Deutsche Forschungsgemeinschaft
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Tuomisto, L., Yamatodani, A., Dietl, H. et al. In vivo release of endogenous catecholamines, histamine and GABA in the hypothalamus of Wistar Kyoto and spontaneously hypertensive rats. Naunyn-Schmiedeberg's Arch. Pharmacol. 323, 183–187 (1983). https://doi.org/10.1007/BF00497660
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DOI: https://doi.org/10.1007/BF00497660