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Electrically evoked noradrenaline release from cultured chick sympathetic neurons: modulation via presynaptic α2-adrenoceptors and lack of autoinhibition

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Summary

Sympathetic neurons from twelve day old chick embryos were plated on polystyrol discs and kept in culture for five days. After incubation with 3H-noradrenaline the discs were transferred to small chambers and superfused. Electrical field stimulation (36 pulses at 3 Hz) increased the outflow of tritium. The evoked overflow of tritium was abolished in the absence of extracellular calcium and was diminished by about 90% in the presence of tetrodotoxin (1 μmol/l). The α2-adrenoceptor agonist 5-bromo-6-(2-imidazolin-2-ylamino)quinoxaline (UK-14,304) caused a concentration-dependent decrease in overflow, whereas the α1-adrenoceptor agonist methoxamine was ineffective at up to 1 μmol/l. The concentration-response curve of UK-14,304 was shifted to the right by the α2-adrenoceptor antagonist yohimbine (0.03 μmol/l). Yohimbine on its own caused no significant change. The noradrenaline reuptake inhibitor cocaine (10 μmol/l) caused a small (20%) increase in evoked overflow. The results indicate that cultured chick sympathetic neurons possess release-modulating α2-adrenoceptors and that the electrically induced overflow of transmitter occurs under conditions virtually free of autoinhibition.

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Authors and Affiliations

  1. Institute of Neuropharmacology, University of Vienna, Wahringer Strasse 13a, A-1090, Vienna, Austria

    S. Böhm & S. Huck

  2. Institute of Pharmacology, University of Vienna, Wahringer Strasse 13a, A-1090, Vienna, Austria

    H. Drobny & E. A. Singer

Authors
  1. S. Böhm
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  2. S. Huck
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  3. H. Drobny
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  4. E. A. Singer
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Böhm, S., Huck, S., Drobny, H. et al. Electrically evoked noradrenaline release from cultured chick sympathetic neurons: modulation via presynaptic α2-adrenoceptors and lack of autoinhibition. Naunyn-Schmiedeberg's Arch Pharmacol 344, 130–132 (1991). https://doi.org/10.1007/BF00167393

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  • DOI: https://doi.org/10.1007/BF00167393

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