Summary
By use of an electromagnetic flowmeter and the inert gas desaturation method 43 comparative measurements of the blood flow were performed in 6 isolated dog kidneys. For the measurements by use of the latter method krypton-85 and xenon-133 were employed, for a fast, and respectively, a slow injection of the indicator into the renal artery. Calculation of the blood flow from the wash-out curves was made by use of the formulas advanced by Kety (1951) and by Zierler (1965).
A good accordance was found between the flowmeter values and those obtained with the desaturation methods. Recirculation and resorption of indicator in fat tissue surrounding the kidney explain that previous examinations in kidneys in situ have resulted in very low perfusion values, when Zierler's formula has been applied. Extrapolation of the final slope to obtain the area of the curve may be a major source of error by calculation of the blood flow with Zierler's formula.
Similar content being viewed by others
References
Andersen, A. M., and J. Ladefoged: Partition coefficient of xenon-133 between various tissues and blood in vivo. Scand. J. clin. Lab. Med. 19, 72 (1967).
Bell, G., and A. M. Harper: Measurements of local blood flow in the renal cortex from clearance of krypton-85. J. Surg. Res. 5, 382 (1965).
Deetjen, P., H. Brechtelsbauer, and K. Kramer: Hämodynamik des Nierenmarks. Pflügers Arch. ges. Physiol. 279, 281 (1964).
Kety, S. S.: The theory and applications of the exchange of inert gas at the lungs and tissue. Pharmacol. Rev. 3, 1 (1951).
Khouri, E., and D. E. Gregg: Miniature electromagnetic flow meter applicable to coronary arteries. J. appl. Physiol. 18, 224 (1963).
Kjellmer, I., I. Lindbjerg, I. Prerovsky, and H. Tonnesen: The relation between blood flow in an isolated muscle measured with the Xe133 clearance and a direct recording technique. Acta physiol. scand. 69, 69–78 (1967).
Ladefoged, J.: Measurements of the renal blood flow in man with the xenon-133 wash-out technique. Scand. J. clin. Lab. Invest. 18, 299 (1966).
Thorburn, G. D., H. H. Kopald, J. A. Herd, M. Hollenberg, C. CC. O'Morchoe, and A. C. Barger: Intrarenal distribution of nutrient blood flow determined with krypton-85 in the uaesthetized dog. Circulat. Res. 13, 290 (1963).
Zierler, K. L. Equations for measuring blood flow by external monitoring of radioisotopes. Circulat. Res. 16, 309 (1965).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ladefoged, J., Pedersen, F., Bassenge, E. et al. Renal blood flow in isolated kidneys measured with an electromagnetic flowmeter and by xenon-133 and krypton-85 wash-out techniques. Pflügers Archiv 299, 30–37 (1968). https://doi.org/10.1007/BF00362539
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00362539
Key-Words
- Radioactive Wash-out Technique
- Renal Blood Flow
- Electromagnetic Flowmeter
- Isolated Kidney
- Inert Gas Desaturation