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Wirkung von Aldosteron auf die Natrium-und Wasserresorption im proximalen Tubulus bei chronischer Kochsalzbelastung

Effect of aldosterone on proximal tubular sodium and water reabsorption in chronically salt loaded rats

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Summary

Rats were kept on isotonic saline as drinking fluid for a period of 4 weeks. Glomerular filtration rate and proximal transit time of Lissamine green remained unchanged. The intrinsic reabsorptive capacity of the proximal tubular epithelium—measured by the “shrinking-droplet-method”—decreased significantly. As a result, fractional proximal reabsorption of sodium and water decreased from 51 to 38 per cent of the filtered load. Sodium excretion rose from 0.06 (controls) to 1,25 per cent of the filtered load. These changes were reversible by injection of d-aldosterone. The results were confirmed by measuring the TF/P ratio of inulin at the end of the proximal convolution.

The results indicate that in rats chronically loaded with salt, the inhibition of fractional proximal reabsorption is due to a decreased secretion of mineralocorticoids, and is the cause of the higher sodium excretion. Apparently, the decreased proximal reabsorption is partly compensated in more distal parts of the nephron.

Zusammenfassung

Ratten erhielten 4 Wochen lang isotone Kochsalzlösung als Trinkflüssigkeit. Glomerulumfiltrat und Passagezeit von Lissamin-Grün im proximalen Tubulus blieben unverändert. Die spezifische Resorptionskapazität des Tubulusepithels — gemessen mit der „gespaltenen-Tropfen-Methode“ — nahm signifikant ab. Daraus ergab sich eine Verminderung der fraktionellen Natrium-und Wasserresorption im proximalen Tubulus von 51 auf 38% der gefilterten Menge. Die Na+-Ausscheidung stieg von 0,06 (Kontrollen) auf 1,25% der gefilterten Menge. Diese Veränderungen waren rückgängig zu machen durch Injektion von d-Aldosteron. Durch Bestimmung des TF/P-Inulin-Quotienten am Ende des proximalen Konvolutes wurden die Ergebnisse bestätigt.

Es wird angenommen, daß die Resorptionshemmung im proximalen Tubulus bei chronischer Kochsalzbelastung durch verminderte Sekretion von Mineralocorticoiden bedingt und letzten Endes Ursache der vermehrten Na+-Ausscheidung ist. Ein Teil der proximalen Resorptionshemmung wird anscheinend in weiter distal gelegenen Nephron-Abschnitten kompensiert.

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  1. Max-Planck-Institut für experimentelle Medizin Abteilung Physiologie, Göttingen

    Klaus O. Stumpe & Bruno Ochwadt

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  1. Klaus O. Stumpe
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Stumpe, K.O., Ochwadt, B. Wirkung von Aldosteron auf die Natrium-und Wasserresorption im proximalen Tubulus bei chronischer Kochsalzbelastung. Pflügers Archiv 300, 148–160 (1968). https://doi.org/10.1007/BF00362546

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