Summary
The hypothesis of renal origin of the humoral factor responsible for the natriuresis which follows saline infusion in the dog was tested in a cross-circulation model especially designed for the purpose. Renal venous blood of saline loaded donor dogs was pumped directly into the system perfusing the right (“assay”) kidney of oliguric recipient animals. In control and recovery periods, recipients own renal venous blood was substituted for the blood of saline-infused donors.
Sodium excretion increased slightly from the control value of 2.8±1.2 (S.D.) to 4.7±3.5 μEq/min (68 per cent increase,p<0.05), but only slight recovery toward control rate was noted within 25 min of cessation of cross-circulation. Simultaneously, filtered sodium decreased slightly from 2.8±1.15 (S.D.) to 2.7±1.10 mEq/min. Glomerular filtration rate (C CR) and effective renal plasma flow (C PAH) decreased gradually during the experiment. Urine flow,U/P osm, hematocrit, and perfusion pressure of the “assay” kidney showed only minor changes.
Since the slight increase in sodium excretion during cross-circulation constituted but a small fraction of natriuresis observed in saline-loaded donors, it was concluded that the results are incompatible with the release from the kidney of saline-infused animals of a potent natriuretic factor.
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This study was supported by American Heart Association Grant 66630.
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Sadowski, J., Morrison, A.L., Selkurt, E.E. et al. Examination of possible renal origin of the humoral factor responsible for saline diuresis in the dog. Pflugers Arch. 312, 99–109 (1969). https://doi.org/10.1007/BF00588535
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DOI: https://doi.org/10.1007/BF00588535