Abstract
Wash out analysis of tracer Na from frog skins after labelling from the epithelial or corial side was performed under control conditions and after transport inhibition by amiloride or ouabain. Wash out from the transport pool, i. e. after labelling from the epithelial side, into the corial bathing solution could be resolved into two individual components under all conditions. The fast one with half times of wash out of about 1.8 min correlates with the rate of transepithelial Na transport. It cannot unequivocally be associated with intra- or extracellular tissue spaces. The slow component with half times of about 18 min is essentially independent of the transport rate. The amount of Na washed out as this component increased considerably after transport inhibition with ouabain, suggesting intracellular location of at least a fraction of the transport pool, which appears to be made up by the entire epithelial cell layers. Wash out of tracer Na from the transport pool to the epithelial side is small compared to wash out to the corial side. Tracer wash out characteristics after labelling from the corial side were similar for wash out to the epithelial and the corial side. This suggests that considerable paracellular fluxes contribute to the Na flux from the corial to the epithelial side. The total intracellular Na present in the epithelium after ouabain can be labelled from the corial side. Carrier mediated Na−Na exchange across the basolateral membranes is proposed to account for this.
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Nagel, W., Moshagen, D. Wash out characteristics of tracer Na from the transport pool of frog skin. Pflugers Arch. 374, 235–241 (1978). https://doi.org/10.1007/BF00585600
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DOI: https://doi.org/10.1007/BF00585600