Abstract
The effect of cooling from 35 to 20° C on the45Ca-exchange and on the contractile response of rabbit ear artery has been investigated.
The amplitude of the contraction induced by K-depolarization at 20° C is reduced to about 60% of its value at 35° C, whereas the response to noradrenaline is not significantly affected.
Cooling induces a 2 to 4-fold reduction of the45Ca-efflux rate. This effect also occurs in Ca-free medium and in solutions containing 1 mM La. It also occurs in Na-free medium and in tissues in which the transmembrane Na-gradient has been reduced.
At 20° C, the45Ca-influx in unstimulated tissues and in K-depolarized preparations is significantly lower than at 35° C. in Ca-depleted tissues, i.e. tissues in which the noradrenaline-sensitive Ca-store has been emptied by a stimulation with the agonist in Ca-free solution, the45Ca-influx is not significantly affected by cooling.
The gradual depletion of the noradrenaline-sensitive Ca-store in Ca-free solutions is at 20° C much slower than at 35° C. The amount of Ca released by noradrenaline is not affected by cooling, whereas for the same amount of Ca released the contractile response is higher at 20° C.
These findings indicate that temperature affects the transmembrane Ca-extrusion and the Ca-influx through voltage-dependent channels. The properties of the noradrenaline-sensitive Ca-store are less sensitive to temperature.
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References
Aprigliano O, Hermsmeyer K (1976) In vitro denervation of the portal vein and caudal artery of the rat. J Pharmacol Exp Ther 198:568–577
Blaustein MP (1977) Sodium ions, calcium ions, blood pressure regulation and hypertension: A reassessment and an hypothesis. Am J Physiol 232:C165-C173
Bolton TB (1979) Mechanism of action of transmitters and other substances on smooth muscle. Physiol Rev 59:606–718
Bohr DF, Filo RS, Guthe KF (1962) Contractile protein in vascular smooth muscle. Physiol Rev 42, Suppl 5:98–107
Casteels R, Droogmans G (1981) Exchange characteristics of the noradrenaline sensitive Ca store in vascular smooth muscle of rabbit ear artery. J Physiol (Lond) 317:263–279
Chapman D (1975) Fluidity and phase transitions of cell membranes. Biomembranes 7:1–9
Deth R, Van Breemen C (1977) Agonist induced release of intracellular Ca2+ in the rabbit aorta. J Membr Biol 30:363–380
Devine CE, Somlyo AV, Somlyo AS (1972) Sarcoplasmic reticulum and excitation-contraction coupling in mammalian smooth muscle. J Cell Biol 52:690–718
Droogmans G, Casteels R (1979) Sodium and calcium interactions in vascular smooth muscle cells of the rabbit ear artery. J Gen Physiol 74:57–70
Droogmans G, Casteels R (1980) Effect of temperature on45Ca efflux and contractile response to norepinephrine in vascular smooth muscle. Proc Internat Un Physiol Sci, vol XIV, p 387
Droogmans G, Raeymaekers L, Casteels R (1977) Electro- and pharmacomechanical coupling in the smooth muscle cells of the rabbit ear artery. J Gen Physiol 70:129–148
Glover WE, Strangeways DH, Wallace WFM (1968) Responses of isolated ear and femoral arteries of the rabbit to cooling and to some vasoactive drugs. J Physiol (Lond) 194:78P-79P
Goodford PJ (1965) The loss of radioactive45calcium from the smooth muscle of the guinea-pig taenia coli. J Physiol (Lond) 176:180–190
Inesi G, Millman M, Eletr S (1973) Temperature-induced transitions of function and structure in sarcoplasmic reticulum membranes. J Mol Biol 81:483–504
Lee KS, Shin BC (1969) Studies on the active transport of calcium in human red cells. J Gen Physiol 54:713–729
Peiper U, Griebel L, Wende W (1971a) Unterschiedliche Temperaturabhängigkeit der Gefäßmuskelkontraktion nach Aktivierung durch Kalium-Depolarization bzw. Noradrenalin. Pflügers Arch 324:67–78
Peiper U, Griebel L, Wende W (1971b) Activation of vascular smooth muscle of rat aorta by noradrenaline and depolarization: Two different mechanisms. Pflügers Arch 330:74–89
Peiper U, Laven R, Ehl M (1975) Force velocity relationships in vascular smooth muscle. The influence of temperature. Pflügers Arch 356:33–45
Reuter H, Seitz N (1968) The dependence of calcium efflux from cardiac muscle on temperature and external ion composition. J Physiol (Lond) 195:451–470
Somlyo AP, Devine CE, Somlyo AV, North SR (1971) Sarcoplasmic reticulum and the temperature-dependent contraction of smooth muscle in calcium free solution. J. Cell Biol 51:722–741
Van Breemen C (1969) Blockade of membrane calcium fluxes by lanthanum in relation to vascular smooth muscle contractility. Arch Int Physiol Biochim 77:710–716
Van Breemen C (1977) Calcium requirement for activation of intact aortic smooth muscle. J Physiol (Lond) 272:317–329
Vanhoutte PM, Lorenz RR (1970) Effect of temperature on reactivity of saphenous, mesenteric and femoral veins of the dog. Am J Physiol 218:1746–1750
Vanhoutte PM, Shepherd JT (1970) Effect of temperature on reactivity of isolated cutaneous veins of the dog. Am J Physiol 218:187–190
Villamil MF, Rettori V, Yeyati N (1973) Calcium exchange and distribution in the arterial wall. Am J Physiol 224:1314–1319
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Droogmans, G., Casteels, R. Temperature-dependence of45Ca fluxes and contraction in vascular smooth muscle cells of rabbit ear artery. Pflugers Arch. 391, 183–189 (1981). https://doi.org/10.1007/BF00596168
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DOI: https://doi.org/10.1007/BF00596168