Abstract
To characterize the chloride conductance of human sweat duct the effect of various analogues of diphenylamine-2-carboxylate was investigated on the transepithelial potential difference (PDT) and resistance (R T ) of isolated microperfused sweat ducts. Although the most powerful analogues which block Cl− channels in various secretory and absorptive epithelia were ineffective, a number of analogues (in particular Cl substituted ones) were found which at high concentrations significantly and reversibly increased PDT andR T . The data suggest that the main chloride conductance pathway of sweat duct epithelium resides in the cell membranes rather than in the tight junctions. In addition the different blocking spectra of the chloride conductances of sweat duct and tracheal epithelium (Welsh MJ, Science 232:1648, 1986) suggest that the combined impairment of both conductances in cystic fibrosis does not result from a molecular defect in the Cl− channels.
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References
Balaban RS, Mandel LJ, Benos DJ (1979) On the cross reactivity of amiloride and 2,4,6-triaminopyrimidine (TAP) for the cellular entry and tight junctional cation permeation pathways in epithelia. J Membr Biol 49:363–390
Bentley PJ (1968) Amiloride a potent inhibitor of Na transport across the toad bladder. J Physiol 195:317–330
Bijman J, Frömter E (1986) Direct demonstration of high transepithelial chloride-conductance in normal human sweat duct which is absent in cystic fibrosis. Pflügers Arch 407 (Suppl 2): S123-S127
Cragoe EJ, Gould NP, Woltersdorfer OW, Ziegler C (1982) Agents for the treatment of brain injury. 1. (Aryloxy)alkanoic acids. J Med Chem 25:567–579
Di Stefano A, Wittner M, Schlatter E, Lang HJ, Englert H, Greger R (1985) Diphenylamine-2-carboxylate, a blocker of the Cl-conductive pathway in Cl-transporting epithelia. Pflügers Arch 405:S95-S100
Emrich HM, Ullrich KJ (1966) Ausscheidung verschiedener Stoffe in Schweiß in Abhängigkeit von der Schweißflußrate. Pflügers Arch 290:298–310
Foskett JK, Ussing HH (1986) Localization of chloride conductance to mitochondria-rich cells in frog skin epithelium. J Membr Biol 91:251–258
Frizzell RA, Rechkemmer G, Shoemaker RL (1986) Altered regulation of airway epithelial cell chloride channels in cystic fibrosis. Science 233:558–560
Greger R (1981) Cation selectivity of the isolated perfused cortical thick ascending limb of Henle's loop of rabbit kidney. Pflügers Arch 390:30–37
Greger R, Schlatter E, Gögelein H (1985) Cl− channels in the apical cell membrane of the rectal gland “induced” by cAMP. Pflügers Arch 403:446–448
Kottra G, Frömter E (1984) Multi-site action of amiloride on Necturus gallbladder transepithelial resistance. Pflügers Arch 400:R26
Lichton IJ (1957) Osmotic Pressure of human sweat. J Appl Physiol 11:422–424
Moreno JH (1975) Blockage of gallbladder tight junction cation-selective channels by 2,4,6-triaminopyrimidinium (TAP). J Gen Physiol 66:97–115
Quinton PM (1981) Effects of some transport inhibitors on secretion and reabsorption in intact and perfused single human sweat glands. Pflügers Arch 391:309–313
Quinton PM (1983) Chloride impermeability in cystic fibrosis. Nature 381:421–422
Quinton PM, Bijman J (1983) Higher bioelectric potentials in sweat glands due to decreased Cl absorption in patients with cystic fibrosis. N Engl J Med 308:185–189
Reddy MM, Quinton PM (1986) Intracellular microelectrode analysis of chloride transport in normal and cystic fibrosis human reabsorptive sweat ducts. Biophys J 49:157a
Sato K (1986) Beta-adrenergic dysfunction of sweat secretion. FASEB-70th Annual Meeting, St. Louis, MO, USA
Sato K, Sato F (1984) Defective β-adrenergic response of cystic fibrosis sweat glands in vivo and in vitro. J Clin Invest 73:1763–1771
Schulz IJ, Frömter E (1968) Mikropunktionsuntersuchungen an Schweissdrüsen von Mucoviscidosepatienten und gesunden Versuchspersonen. In: Windorfer H, Stephan U (eds) Mucoviscidose. Thieme, Stuttgart, pp 12–21
Wangemann P, Wittner M, Di Stefano A, Englert HC, Lang HJ, Schlatter E, Greger R (1986) Cl−-channel blockers in the thick ascending limb of the loop to Henle. Structure activity relationship. Pflügers Arch 407:S128-S141
Welsh MJ (1986) An apical-membrane chloride channel in human tracheal epithelium. Science 232:1648–1650
Welsh MJ, Liedtke CM (1986) Chloride and potassium channels in cystic fibrosis airway epithelia. Nature 322:467–470
Widdicombe JH, Welsh MJ, Finkbeiner WE (1985) Cystic fibrosis decreases the apical membrane chloride permeability of monolayers cultures from cells of tracheal epithelium. Proc Natl Acad Sci USA 82:6167–6171
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Bijman, J., Englert, H.C., Lang, H.J. et al. Characterization of human sweat duct chloride conductance by chloride channel blockers. Pflugers Arch. 408, 511–514 (1987). https://doi.org/10.1007/BF00585077
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DOI: https://doi.org/10.1007/BF00585077