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Kinetic behavior of penicillin acylase immobilized on acrylic carrier

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Abstract

The usefulness of Lilly's kinetic equation to describe penicillin G hydrolysis performed by immobilized penicillin acylase onto the acrylic carrier has been shown. Based on the experimental results characteristic kinetic constants have been estimated. The effect of noncompetitive inhibition of 6-amino penicillanic acid has not been found. Five components of reaction resistance have been defined. These components were also estimated for the reaction of the native enzyme as well as the Boehringer preparation.

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Abbreviations

C E g/m3 :

enzyme concentration

C P,C Q mol/m3 :

product concentrations

C S mol/m3 :

substrate concentration

C SO mol/m3 :

initial substrate concentration

K A mol/m3 :

constant which defines the affinity of a substrate to the enzyme

K iS mol/m3 :

substrate inhibitory constant

K iP mol/m3 :

PhAA inhibitory constant

K iQ mol/m3 :

6-APA inhibitory constant

k 3 mol/g/min:

constant rate of dissociation of the active complex

R(1):

concentrational component of reaction resistance

R(2):

resistance component derived from substrate affinity

R(3):

resistance component due to the inhibition of the enzyme by substrate

R(4):

resistance component due to the inhibition of the enzyme by PhAA

R(5):

resistance component due to inhibition of the enzyme by 6-APA

r = dCs/dt mol/m3 min:

rate of reaction

t :

min reaction time

υ(i):

relative resistance of reaction

References

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Authors and Affiliations

  1. Institute of Chemical Engineering, Technical University of Wroclaw, Norwida 4/6, 51-373, Wroclaw, Poland

    J. Bryjak & A. Noworyta

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  1. J. Bryjak
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  2. A. Noworyta
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Bryjak, J., Noworyta, A. Kinetic behavior of penicillin acylase immobilized on acrylic carrier. Bioprocess Engineering 9, 37–42 (1993). https://doi.org/10.1007/BF00389538

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  • DOI: https://doi.org/10.1007/BF00389538

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