Zusammenfassung
Die Substanzen werden extrahiert, auf Silicagel-Dünnschichten getrennt und mit Natronlauge besprüht, wodurch sie fluorescierend werden. Sevin wird dabei in α-Naphthol umgewandelt. Die Fluorescenz der beiden Flecke wird bei 488 nm gemessen (Anregung: 338 nm). Die Nachweisgrenze liegt visuell bei 0,006 μg je Fleck, bei instrumenteller Messung bei 0,001 μg. Reproduzierbarkeit und Genauigkeit des Verfahrens sind der Gas-Chromatographie und anderen Methoden vergleichbar.
Summary
A fluorimetric in situ method is described for the determination of Sevin and α-naphthol. The compounds are extracted, separated on silicagel-gel thin layers and sprayed with NaOH solution to render them fluorescent. Sevin is converted to α-naphthol by this treatment. The fluorescence of both spots is measured at 488 nm (excitation: 338 nm). Visual detection limit is 0.006 μg/spot, by instrumental measurement it is 0.001 μg/spot. Reproducibility and accuracy are comparable to gas chromatography and other methods.
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Frei, R.W., Lawrence, J.F. & Belliveau, P.E. An in situ fluorimetric method for the determination of Sevin and α-naphthol on thin-layer chromatograms. Z. Anal. Chem. 254, 271–274 (1971). https://doi.org/10.1007/BF00428760
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DOI: https://doi.org/10.1007/BF00428760