Conclusion
All of the immunoassays tested were rapid and easy to perform. The precision of these tests was adequate and the results were comparable with those obtained by HPLC. The EMIT correlated best with HPLC and showed a high specificity. Normally encountered levels of other xanthines or uric acids apparently do not interfere with this assay.
References
Oellerich M (1980) In: Wettengel R (ed) Asthma-Therapie mit Theophyllin Optimierung durch Blutspiegel-Bestimmung. Dustri-Verlag Dr. Karl Feistle, München-Deisenhofen, pp 66–82
Staib AH, Schuppan D, Lissner R, Zilly W, v Bomhard G, Richter E (1980) Internat J Clin Pharmacol Therap Toxicol 18:500–502
Jusko WJ, Poliszczuk A (1976) Am J Hosp Pharm 33:1193–1196
Schuster R, Riedmann M, Zech K (1982) Hewlett Packard application note 232-21, in press
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Oellerich, M., Külpmann, W.R., Beneking, M. et al. Determination of theophylline in serum by non-isotopic immunoassays (EMIT, SLFIA, NIIA) and HPLC — A comparative study. Z. Anal. Chem. 311, 355–356 (1982). https://doi.org/10.1007/BF00481724
Issue Date:
DOI: https://doi.org/10.1007/BF00481724