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Quantitative photometrische Bestimmung der Pyrrolizidinalkaloide in Symphity Radix

Quantitative photometric determination of pyrrolizidine alkaloids in Symphyti Radix

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Summary

A colour reaction for unsaturated pyrrolizidine alkaloids (PAs), which has already been published, is used for spectrophotometric determination of the toxic PAs in Symphyti Radix (Symphytum officinale L., Boraginaceae). By changing several parameters it was possible to reduce the detection limit to 1 microgram and to improve significantly the reproducibility of the quantitative determination. For Symphyti Radix a simple extraction method allowing the detection of 10 ppm PAs in 1 gram dried drug (0.001%) was validaded.

Zusammenfassung

Eine bereits in der Literatur beschriebene Farbreaktion für 1,2-ungesättigte Pyrrolizidinalkaloide (PAs) wird zur Gehaltsbestimmung der toxischen PAs in Symphyti Radix herangezogen. Durch Veränderung einiger Versuchsparameter konnte die Erfassungsgrenze auf 1 Mikrogramm herabgesetzt und die Reproduzierbarkeit der Messung erheblich verbessert werden. Für die alkaloidreichen Beinwellwurzeln wurde eine einfache Extraktionsmethode validiert, die einen Nachweis von 10 ppm in einem Gramm getrockneter Droge gestattet (0,001%).

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Literatur

  1. Koehler H, Franz G (1987) Z Phytotherapie 8:166–168

    Google Scholar 

  2. Wichtel M (1989) Teedrogen. Wissenschaftliche Verlags GmbH, Stuttgart, pp 88–90

    Google Scholar 

  3. Graza L, Koch H, Löffler E (1985) Arch Pharm 318:1090–1095

    Google Scholar 

  4. Andrès R (1991) Thesis, Bern

  5. Roeder E (1984) Pharm Zeit 13:33–38

    Google Scholar 

  6. Furuya T, Araki K (1968) Chem Pharm Bull 16:2512–2516

    Google Scholar 

  7. Furuya T, Hikichi M (1971) Phytochemistry 10:2217–2220

    Google Scholar 

  8. Stengel P, Wiedenfeld H, Roeder E (1982) Dtsch Apoth Z 122:851–855

    Google Scholar 

  9. Huizing HJ (1985) Thesis, Groningen

  10. Roeder E, Neuberger V (1988) Dtsch Apoth Z 128:1991–1994

    Google Scholar 

  11. Roeder E, Boureaul T, Neuberger V (1990) unveröffentlichte Ergebnisse

  12. Hirono I, Mori H, Haga M (1978) J Nat Cancer Inst 61:865–869

    Google Scholar 

  13. Hirono I, Haga M, Fujii M, Matsuura S, Matsubara N et al (1979) J Nat Cancer Inst 63:469–471

    Google Scholar 

  14. Anonym (1988) Bundesgesundheitsamt, Berlin, Erlaß v. 10.8.1988 (Az G V 7 7251-01-25365/1)

  15. Bundesgesundheitsamt, Berlin (1990) BAnz Nr. 138:3866 v. 27.7.1990

  16. van Urk HW (1929) Pharm Weekbl 66:473–481

    Google Scholar 

  17. Mattocks AR (1967) Anal Chem 39:443–447

    Google Scholar 

  18. Mattocks AR (1968) Anal Chem 40:1749–1750

    Google Scholar 

  19. Bingley JB (1968) Anal Chem 40:1166–1167

    Google Scholar 

  20. Polonovski MM (1927) Bull Soc Chim Fr 41:1190–1208

    Google Scholar 

Download references

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Authors and Affiliations

  1. Pharmazeutisches Institut der Universität Bonn, An der Immenburg 4, W-5300, Bonn 1, Bundesrepublik Deutschland

    Erhard Roeder & Ke Liu

  2. Department of Pharmacy, Liaoning College of Traditional Chinese Medicine, Shenyang, People's Republic of China

    Ke Liu

  3. Institut für Botanik und Pharmazeutische Biologie, Staudtstrasse 5, W-8520, Erlangen, Bundesrepublik Deutschland

    Reinhard Mütterlein

Authors
  1. Erhard Roeder
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  2. Ke Liu
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  3. Reinhard Mütterlein
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Roeder, E., Liu, K. & Mütterlein, R. Quantitative photometrische Bestimmung der Pyrrolizidinalkaloide in Symphity Radix. Fresenius J Anal Chem 343, 621–624 (1992). https://doi.org/10.1007/BF00324124

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  • DOI: https://doi.org/10.1007/BF00324124

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