Summary
A quantitative ultrastructural survey was made of subsurface cisterns and their association with overlying structures in the left hypoglossal nucleus of normal rats, and rats which had received left hypoglossal axotomies 7–84 days previously.
Subsurface cisterns in normal rats occurred in some hypoglossal neurones, and, sporadically, in proximal dendrites. They were mostly subsynaptic, and often associated with Nissl substance. From 7–14 days postoperatively, when many somatic boutons temporarily lost contact with the perikaryal surface, and were replaced by a microglial sheath, the percentage of perikaryon with underlying cistern was significantly reduced. The Nissl substance was also dispersed at this stage, and not restored until 28 days postoperatively. At 21 days normal percentages of subsurface cistern were restored, but the cisterns were now mostly subastrocytic, an astrocytic sheath having replaced the microglial sheath. From 63 days onwards the cisterns were mostly subsynaptic again as boutons returned to the regenerating perikarya and the temporary astrocytic sheath disappeared.
It is suggested that subsurface cisterns might alter the overlying perikaryal surface in some way during neuronal regeneration, causing certain boutons to adhere there.
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References
Bodian, D.: A suggestive relationship of nerve cell RNA with specific synaptic sites. Proc. nat. Acad. Sci. (Wash.) 53, 418–425 (1965)
Bodian, D.: Synaptic diversity and characterization by electron microscopy. In: Structure and Function of Synapses, pp. 45–66. Eds. by G. D. Pappas and D. P. Purpura. New York: Raven Press 1972
Davidoff, M.: Über die Glia im Hypoglossuskern der Ratte nach Axotomie. Z. Zellforsch. 141, 427–442 (1973)
Flock, Å.: Transducing mechanisms in the lateral line organ receptors. Cold Spr. Harb. Symp. quant. Biol. 30, 133–145 (1965)
Hartmann, J.F.: Ultrastructural relationships of neuronal cytoplasmic membranes. Med. biol. Ill. 16, 109–113 (1966)
Herndon, R.M.: The fine structure of the Purkinje cell. J. Cell. Biol. 18, 167–180 (1963)
Kerns, J.M., Kinsman, E.J.: Neuroglial response to sciatic neurectomy. II. Electron Microscopy. J. comp. Neurol. 151, 255–280 (1973)
Pappas, G.D., Purpura, D.P.: Fine structure of dendrites in the superficial neocortical neuropil. Exp. Neurol. 4, 507–530 (1961)
Pappas, G.D., Waxman, S.G.: Synaptic fine structure — morphological correlates of chemical and electrotonic transmission. In: Structure and Function of Synapses, pp. 10–13. Eds. by G. D. Pappas and D. P. Purpura. New York: Raven Press 1972
Rosenbluth, J.: The fine structure of acoustic ganglia in the rat. J. Cell. Biol. 12, 329–359 (1962a)
Rosenbluth, J.: Subsurface cisterns and their relationship to the neuronal plasma membrane. J. Cell Biol. 13, 405–421 (1962b)
Rosenbluth, J., Palay, S.L.: Electron microscopic observations on the interface between neurons and capsular cells in the dorsal root ganglia of the rat. Anat. Rec. 136, 268 (1960)
Siegesmund, K.A.: The fine structure of subsurface cisterns. Anat. Rec. 162, 187–196 (1968)
Smith, C.A., Sjöstrand, F.S.: Structure of the nerve endings of the external hair cells of the guinea pig cochlea as studied by serial sections. J. Ultrastruct. Res. 5, 523–556 (1961)
Sotelo, C., Palay, S.L.: The fine structure of the lateral vestibular nucleus in the rat. I. Neurons and neuroglial cells. J. Cell Biol. 36, 151–179 (1968)
Sumner, B.E.H.: The nature of the dividing cells around axotomized hypoglossal neurones. J. Neuropath. exp. Neurol. in press (1974)
Sumner, B.E.H., Sutherland, F.I.: Quantitative electron microscopy on the injured hypoglossal nucleus in the rat. J. Neurocytol. 2, 315–328 (1973)
Watson, W.E.: An autoradiographic study of the incorporation of nucleic-acid precursors by neurons and glia during nerve regeneration. J. Physiol. (Lond.) 180, 741–753 (1965)
Wersäll, J., Flock, Å., Lundquist, P.-G.: Structural basis for directional sensitivity in cochlear and vestibular sensory receptors. Cold Spr. Harb. Symp. quant. Biol. 30, 115–132 (1965)
Whittaker, V.P., Gray, E.G.: The synapse: biology and morphology. Brit. med. Bull. 18, 223–228 (1962)
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Sumner, B.E.H. A quantitative study of subsurface cisterns and their relationships in normal and axotomized hypoglossal neurones. Exp Brain Res 22, 175–183 (1975). https://doi.org/10.1007/BF00237687
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DOI: https://doi.org/10.1007/BF00237687