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A selective tachykinin receptor agonist promotes differentiation but not survival of rat chromaffin cells in vitro

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Summary

Rat chromaffin cells display phenotypic plasticity postnatally. In the presence of glucocorticoids, they retain a chromaffin cell phenotype, whilst in the presence of nerve growth factor and the absence of glucocorticoids they adopt a sympathetic neuronal phenotype. The tachykinins have some of the characteristics of a neurotrophic factor and are present in the form of a substance P afferent input in the rat adrenal medulla. We investigated the effects of stable NK1 and NK2 tachykinin receptor agonists, Ava[L-Pro,N-Me-Leu-10]SP7-11 (GR73632) and [Lys-3,Gly-8,R-Lac-Leu-9]NKA3-10 (GR64349), respectively, on the survival and phenotype of P5-7 rat chromaffin cells in vitro. GR73632 promoted neurite outgrowth, characteristic of the sympathetic neuronal phenotype, in the absence of NGF and glucocorticoids, but was without effect on survival after 2 weeks in culture. GR64349 was without effect.

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Authors and Affiliations

  1. MRC Cambridge Brain Repair Centre and Departments of Experimental Psychology, University of Cambridge, CB2 3EB, Cambridge, UK

    Roger Barker & Stephen Dunnett

  2. MRC Cambridge Brain Repair Centre and Department of Physiology, University of Cambridge, CB2 3EB, Cambridge, UK

    Roger Barker & James Fawcett

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  1. Roger Barker
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  2. Stephen Dunnett
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  3. James Fawcett
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Barker, R., Dunnett, S. & Fawcett, J. A selective tachykinin receptor agonist promotes differentiation but not survival of rat chromaffin cells in vitro. Exp Brain Res 92, 467–472 (1993). https://doi.org/10.1007/BF00229034

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  • DOI: https://doi.org/10.1007/BF00229034

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