Abstract
The tissue and sub-cellular distribution of Fe, Cu, Zn and the naturally occurring radionuclide polonium-210 was determined in the gastropod mollusc Haliotis rubra collected from Western Port Bay, Australia, between March and July 1988. The highest concentrations of the metals, with the exception of Cu, were found in the digestive gland. Copper was more uniformly distributed, with tissues that are more vasculated having higher concentrations. Ultrastructural examination of the digestive gland, gill and kidney showed dense membrane-bound granules within the cytoplasm. Elemental analysis of the granules by electron probe x-ray microanalysis indicated that the granules in the digestive gland and gill contained high concentrations of iron, with small amounts of copper and zinc. In contrast, the metal-containing granules in the kidney were predominantly composed of iron, copper and phosphorus, with variable contributions of sodium potassium, and calcium. Homogenisation and fractionation of the digestive gland by differential centrifugation confirmed that approximately 80, 10, 90 and 50% of the total homogenate Fe, Cu, Zn and 210Po, respectively, sedimented at 1200xg. In the haemolymph, all the elements studied were associated with the soluble high molecular weight component of the serum, not with the amoebocytes. 210Po was present in the mucus-secreting hypobranchial glands at about half the concentration found in gill tissue.
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Aisen, P., Listowsky, I. (1980). Iron transport and storage proteins. A. Rev. Biochem. 49: 357–393
Al-Mohanna, S. Y., Nott, J. A. (1989). Functional cytology of the hepatopancreas of Penaeus semisulcatus (Crustacea: Decapoda) during the moult cycle. Mar. Biol. 101: 535–544
Bacon, M. P., Spencer, D. W., Brewer, P. G. (1976). 210Pb/226Ra 210Po/210Pb disequilibria in seawater and suspended particulate material. Earth planet Sci. Lett. 32: 277–296
Bagnall, K. W. (1973). The chemistry of selenium, tellurium and polonium. In: Schmidt, M., Siebert, W., Bagnall, K. W. (eds.) The chemistry of sulphur, selenium, tellurium and polonium. Pergamon Press, Oxford, p. 935–1008
Bryan, G. W., Hummerstone, L. G. (1973) Brown seaweed as an indicator of heavy metals in estuaries in south-west England. J. mar. biol. Ass. U.K. 53: 705–720
Bryan, G. W., Potts, G. W., Forster, G. R. (1977) Heavy metals in the gastropod mollusc Haliotis tuberculosa. J. mar. biol. Ass. U.K. 57: 379–390
Bullough, W. S. (1958). Practical invertebrate anatomy. Macmillan Press, London
Cherry, R. D., Dowdle, E. B. D., Todd, G. (1979). Intracellular sites of natural 210Po in the lobster hepatopancreas. S. Afr. J. Sci. 75: p. 39
Cherry, R. D., Heyraud, M., Higgio, J. J. W. (1983). Polonium-210: its relative enrichment in the hepatopancreas of marine invertebrates. Mar Ecol. Prog. Ser. 13: 229–236
Crofts, D. R. (1929). Haliotis. L. M. B. C. Mem. typ. Br. mar. Pl. Anim. 29: 1–174
Dales, R. P. (ed) (1981). Practical invertebrate zoology. Blackwell Scientific Publications, London
De Meio, R. H., Yu-Chem Lin, Narasimhulu, S. (1967). Some aspects of the biosynthesis of mactin. Comp. Biochem. Physiol. 20: 581–591
Ellerton, H. D., Lankovsky, T. (1983). Structure of the haemocyanin from pau (abalone) Haliotis iris. Life Chem. Rep. (Suppl.) 1: 129–132
Fisher, N. S., Smith, J. D. (1987). Molecular association of Cu, Zn, Cd and 210Po in the digestive gland of the squid Nototodarus gouldi. Mar. Biol 95: 87–91
Fisher, N. S., Burns, K. A., Cherry, R. D., Heyraud, M. (1983). Accumulation and cellular distribution of 241Am, 210Po and 210Pb in two marine algae. Mar. Ecol. Prog. Ser. 11: 233–237
Folsom T. R., Wong, K. M., Hodge, V. F. (1974). Some extreme accumulations of natural polonium radioactivity observed in certain occanic organisms. In: Adams, J. A. S., Lowder, W. M., Gesell, T. F. (eds) The natural radiation environment. II.Texas University Press, Houston, Texas, p. 863–882
Fox, D. L. (1966). Pigmentation in molluscs. In: Wilbur, K. M., Yonge, C. M. (eds.) Physiology of Mollusca. Vol. 2. Academic Press, New York, p. 249–274
Guary, J. C., Negrel, R. (1981). Calcium phosphate granules: a trap for transuranics and iron in crab hepatopancreas. Comp. Biochem. Physiol. 68A: 423–427
Hamilton, T. F., Smith, J. D. (1986). Improved alpha energy resolution for the determination of polonium isotopes by alpha-spectrometry. Appl. Radiat. Isotopes 37: 628–630
Heyraud, M., Cherry, R. D., Dowdle, E. B. (1987). The subcellular localisation of natural 210Po in the hepatopancreas of the rock lobster (Jasus lalandii) J. envirl Radioact. 5: 249–260
Heyraud, M., Domanski, P., Cherry, R. D., Fasham, M. J. R. (1988). Natural tracers in dietary studies: data for 210Po and 210Pb in decapod shrimp and other pelagic organisms in the Northeast Atlantic Ocean. Mar. Biol. 97: 507–529
Hunt, S., Jevons, F. R. (1966). The hypobranchial mucin of the whelk Buccinum undatum L. Biochem. J. 98: 522–529
Kawai, K. (1961). Comparative biochemical studies on cytochromes and related substances of invertebrates. II. Cytochrome-like haemoproteins in the gut fluids of mulluscs. Biochim. biophys. Acta 52: 241–247
Pilson, M. E. (1965). Variation of hemocyanin concentration in the blood of four species of Haliotis. Biol. Bull. mar. biol. Lab., Woods Hole 128: 459–472
Reynold, E. S. (1963). The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Cell Biol. 17: 209–212
Simkiss, K., Mason, A. Z. (1983). Metal ions: metabolic and toxic effects. In: Hochachka, O. W. (ed.) The Mollusca. Vol 2. Academic Press, New York, p. 101–164
Smith, J. D., Hamilton, T. F. (1984). Improved technique for recovery and measurement of polonium-210 from environmental materials. Analytica chim. Acta 160: 69–77
Smith, J. D., Plues, L., Heyraud, M., Cherry, R. D. (1984). Concentrations of the elements Ag, Al Ca, Cd, Cu, Fe, Mg, Mn, Pb and Zn and the radionuclides 210Pb and 210Po in the digestive gland of the squid Nototodarus gouldii. Mar. envirl Res. 13: 55–68
Spurr, A. R. (1969). A low-viscosity epoxy-resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26: 31–43
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Communicated by G.F. Humphrey, Sydney
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Hyne, R.V., Smith, J.D. & Ellender, G. Tissue and sub-cellular distribution of Fe, Cu, Zn and 210Po in the abalone Haliotis rubra . Marine Biology 112, 75–80 (1992). https://doi.org/10.1007/BF00349730
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DOI: https://doi.org/10.1007/BF00349730