Summary
Gas chromatographic methods are described for the assay of amobarbital and pentobarbital in 500 µl samples of plasma, in concentrations down to 250 ng/ml. After ether extraction at pH 5.5, the barbiturates are reextracted into an alkaline solution of trimethylanilinium hydroxide and are determined quantitatively by gas chromatography as their dimethylated derivatives. The method has been used successfully in volunteers receiving therapeutic doses of these bariturates.
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Ehrnebo, M., Agurell, S. & Boréus, L.O. Gas chromatographic determination of therapeutic levels of amobarbital and pentobarbital in plasma. Eur J Clin Pharmacol 4, 191–195 (1972). https://doi.org/10.1007/BF00635794
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DOI: https://doi.org/10.1007/BF00635794