Summary
This study was performed employing cytogerontologic analysis to investigate the in vitro proliferative capacity of the fibroblasts of scar and normal dermis obtained from the same individual. Scarderived fibroblasts had a shorter in vitro lifespan, longer cell population doubling time, diminished cell numbers at confluency and lower percentages of cell able to incoporate 3H-thymidine than comparable firoblasts derived from normal dermis. These results may indicate that the growth characteristics of scar-derived fibroblasts resemble those of late-passage levels of normal dermal fibroblasts.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Conway H, Gillette RW, Findlay A (1959) Observations on the behavior of human keloids in vitro. Plast Reconstr Surg 24:229
Conway H, Gillette R, Smith JW, Findlay A (1960) Differential diagnosis of keloids and hypertrophic scars by tissue culture technique with notes on therapy of keloids by surgical excision and decadron. Plast Reconstr Surg 25:117
Cristofalo VJ, Sharf BB (1973) Cellular senescence and DNA synthesis. Exp Cell Res 76:419
Diegelmann RF, Cohen IK, McCoy BJ (1979) Growth kinetics and collagen synthesis of normal skin, normal scar and keloid fibroblasts in vitro. J Cell Physiol 98:341
Goldstein S, Littlefield JW, Soeldner JS (1969) Diabetes mellitus and aging: Diminished plating efficiency of cultured human fibroblasts. Proc Natl Acad Sci USA 64:155
Goldstein S (1969) Lifepsan of cultured cells in progeria. Lancet I:424
Grove RL, Cristofalo VJ (1977) Characterization of the cell cycle of cultured human diploid cells: Effects of aging and hydrocortisone. J Cell Physiol 90:415
Hayflick L, Moorehead PS (1961) The serial cultivation of human diploid cell strains. Exp Cell Res 25:585
Hayflick L (1965) The limited in vitro lifetime of human diploid cell strains. Exp Cell Res 37:614
Kay HEM (1965) How many cell-generations? Lancet II:418
Knapp TR, Daniel JR, Kaplan EN (1977) Pathological scar formation. Am J Pathol 86:47
Martin GM, Sprague CA, Epstein CJ (1970) Replicative life-span of cultured human cells effects of donor's age, tissue, and genotype. Lab Invest 23:86
Ross R, Odland G (1968) Human wound repair: II inflammatory cells, epithelial-mesenchymal interrelations, and fibrogenesis. J Cell Biol 39:152
Russell JD, Witt WS (1976) Cell size and growth characteristics of cultured fibroblasts isolated from normal and keloid tissue. Plast Reconstr Surg 57:207
Schneider EL, Braunschweiger K, Mitsui Y (1978) The effect of serum batch on the in vitro lifespans of cell cultures derived from old and young human donors. Exp Cell Res 115:47
Schneider EL, Mitsui Y (1976) The relationship between in cellular aging and in vivo human age. Proc Natl Acad Sci USA 73:3584
Schneider EL (1979) Aging and cultured human skin fibroblasts. J Invest Dermatol 73:15
Wright WE, Hayflick L (1975) Nuclear control of cellular aging demonstrated by hybridization of anucleate and whole cultured normal human fibroblasts. Exp Cell Res 96:113
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yamamoto, M., Tsukada, S. & Inoue, M. Possible age-associated change at cellular level in cultured fibroblasts derived from scar tissue. Chir Plastica 7, 51–58 (1982). https://doi.org/10.1007/BF00266805
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00266805