Summary
Fifty urine samples from a variety of urological patients were analysed using the fluorochrome acridine orange in an automated system. The results were compared with standard colony counts. The method has a potential value in the detection of significant infection particularly in population studies.
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References
Daley RJ, Hobbie JE (1975) Direct counts of aquatic bacteria by a modified epifluorescence technique. Limnol Oceanogr 20:875
Johnston HH, Curtis GDW, Nichols WW (1981) Current states of bioluminescence as a means of detecting significant bacteriuria. Proc 3rd Int Symp Rapid Methods and Automation in Microbiology 162. Tilton RC (ed). Am Soc Microbiol, Washington, DC
Kelly MT, Balfour LC, Latimer JM (1981) Rapid detection and identification of urinary tract pathogens. Proc 3rd Int Symp Rapid Methods and Automation in Microbiology 344. Tilton RC (ed). Am Soc Microbiol, Washington, DC
Kronvall G, Myhre E (1977) Differential staining of bacteria in clinical specimens using acridine orange buffered at low pH. Acta Pathol Microbiol Scand (Section B) 85:249
McCarthy LR, Senne JE (1980) Evaluation of acridine orange stain for detection of microorganisms in blood cultures. Clin Microbiol 11:281
Scholefield J, Manson R, Johnston RJ (1976) The direct staining of microorganisms by selected fluorochromes. Proc 2nd Symp Rapid Methods and Automation in Microbiology 253. Johnston HH, Newson SWB (eds). Learned Information Ltd, Oxford
Spinell M (1982) Bakterienzählung mit dem Bactoscan-Gerät. Deutsche Milchwirtschaft 19
Strugger S (1948) Fluorescence microscope examination of bacteria in soil. Can J Res (Section C) 26:188
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Scholefield, J., Manson, R., Johnston, R.J. et al. The use of acridine orange staining and image analysis to detect bacteriuria. Urol. Res. 13, 141–142 (1985). https://doi.org/10.1007/BF00256076
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DOI: https://doi.org/10.1007/BF00256076