Summary
The present investigation was conducted to examine the combination effect of N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) and N-methyl-N-nitrosourea(MNU) in rat urinary bladder carcinogenesis. Experiment 1 was performed in the groups treated by oral BBN administration in combination with MNU intraversical instillation and their control groups. In the groups given both BBN and MNU, the ratio of rats with the nonpapillary type to carcinoma-bearing rats was significantly higher than in the controls. Since most of the carcinomas were non-invasive, the observation period was prolonged in the groups given both BBN and MNU in experiment 2. However, even after a longer observation period, no invasive carcinoma was observed. These results suggest that high-grade and invasive carcinomas similar to those induced by BBN and MNU in the heterotopically transplanted rat bladder cannot be induced in the natural bladder of the rat.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Babaya K, Takahashi S, Momose H, Matsuki H, Sasaki K, Samma S, Ozono S, Hirao Y, Okajima E (1987) Effects of single chemotherapeutic agents on development of urinary bladder tumor induced by N-butyl-N(4-hydroxybutyl)nitrosamine (BBN) in rats. Urol Res 15:329
Fukushima S, Murasaki G, Hirose M, Nakanishi K, Hasegawa R, Ito N (1982) Histopathological analysis of preneoplastic changes during N-butyl-N-(4-hydroxybutyl)nitrosamine-induced urinary bladder carcinogenesis in rats. Acta Pathol Jpn 32:243
Ito N, Hiasa Y, Tamai A, Okajima E, Kitamura H (1969) Histogenesis of urinary bladder tumors induced by N-butyl-N-(4-hydroxybutyl) nitrosamine in rats. Gann 60:401
Ohtani M, Kakizoe T, Nishio Y, Sato S, Sugimura T, Fukushima S, Niijima T (1986) Sequential changes of invasive carcinomas by N-butyl-N-(4-hydroxybutyl)nitrosamine. Cancer Res 46:2001
Okajima E, Hiramatsu T, Hirao K, Ijuin M, Hirao Y, Babaya K, Ikuma S, Ohara S, Shiomi T, Hijioka T, Ohishi H (1981) Urinary bladder tumors induced by N-butyl-N-(4-hydroxybutyl)nitrosamine in dogs. Cancer Res 41:1958
Oyasu R, Manning DJ, Matsumoto M, Hopp ML (1976) Heterotopic urinary bladder with a communicating reservoir. Cancer Res 36:2261
Oyasu R, Samma S, Ozono S, Bauer K, Wallemark C-B, Homma Y (1987) Induction of high-grade, high-stage carcinomas in the rat urinary bladder. Cancer 59:451
Ozono S, Numata I, Oyasu R (1984) Irreversibility of low-grade superficial rat bladder carcinomas. Cancer Res 44:4260
Samma S, Uemura H, Tabata S, Iwai A, Nakatsuji F, Babaya K, Hirao Y, Okajima E (1984) Rapid induction of carcinoma in situ in dog urinary bladder by sequential treatment with N-methyl-N-nitrosourea and N-butyl-N-(4-hydroxybutyl)nitrosamine. Gann 75:385
Soloway MS (1980) The management of superficial bladder cancer. Cancer 45:1856
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ozono, S., Babaya, K., Sasaki, K. et al. Rat urinary bladder carcinomas induced by N-butyl-N-(4-hydroxybutyl)-nitrosamine and N-methyl-N-nitrosourea. Urol. Res. 18, 323–326 (1990). https://doi.org/10.1007/BF00300780
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00300780