Summary
Although transplanting rat kidneys is an established microsurgical technique, inulin clearance is abnormally low, due to rejection and/or warm ischemia-induced damage. In the present studies, rejection was avoided by using inbred Brown Norway rats as donors and recipients. Donor kidneys were flushed with ice-cold solutions of various composition (saline, saline + 200 or 400 mM mannitol) and pHs (5.7, 6.4, and 7.4), and the kidneys were kept cold during transplantation into unilaterally nephrectomized recipients. Renal function was assessed by clearance techniques 1 week later. In control rats, with both native kidneys intact, the ratio of inulin clearance, left kidney to right kidney, was 0.99±0.02. In rats with a native right kidney and a transplanted left kidney that had been flushed with saline, the ratio was considerably lower (0.46±0.09). Adding 200 mM mannitol to the saline flush solution increased the ratio (0.89±0.09). In comparison, adding 200 mM mannitol and 5 mM phosphate buffer at pH 7.4 resulted in a somewhat lower ratio (0.80±0.09), whereas adding 200 mM mannitol and 5 mM phosphate buffer at pH 5.7 resulted in a higher ratio, one that was indistinguishable from control (0.97±0.09). Thus, in this latter group, the inulin clearances of the transplanted kidneys were identical to those of the contralateral native kidneys.
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References
Andrews PM, Bates SB (1985) Improving Euro-Collins flushing solution's ability to protect kidneys from normothermic ischemia. Mineral Electrolyte Metab 11:309
Andrews PM, Coffey AK (1982) Factors that improve the preservation of nephron morphology during cold storage. Lab invest 46:100
Andrews PM, Coffey AK (1983) Protection of kidneys from acute renal failure resulting from normothermic ischemia. Lab Invest 49:87
Bianchi G, Fox U, DiFrancesco GF, Giovanetti AM, Pagetti D (1974) Blood pressure changes produced by kidney cross-transplantation between spontaneously hypertensive rats and normotensive rats. Clin Sci Mol Med 47:435
Churchill PC, Churchill MC, McDonald FD (1978) Renin secretion and distal tubule Na+ in rats. Am J Physiol 235:F611
Coffman TM, Yarger WE, Klotman PE (1985) Functional role of thromboxane production by acutely rejecting renal allografts in rats. J Clin Invest 75:1242
Coffman TM, Sanfilippo FP, Brazy PC, Yarger WE, Klotman PE (1986) Bilateral native nephrectomy improves renal isograft function in rats. Kidney Int 30:20
Dahl LK, Heine M (1975) Primary role of renal homografts in setting chronic blood pressure levels in rats. Circulation Res 36:692
Donohoe JF, Venkatachalam MA, Bernard DB, Levinski NG (1978) Tubular leakage and obstruction after renal ischemia: structural functional correlations. Kidney Int 13:208
Duncan JI, Heys SD, Thomson AW, Simpson JG, Whiting PH (1988) Influence of the hepatic drug-metabolizing enzyme-inducer phenobarbitone on cyclosporine nephrotoxicity and hepatotoxicity in renal-allografted rats. Transplantation 45:693
Gottschalk CW (1979) Renal nerves and sodium excretion. Ann Rev Physiol 41:229
Jacobsen IA, Pegg DE (1981) Kidney. In: Karow AM Jr, Pegg DE (ed) Organ preservation for transplantation. Dekker, New York, p 553
Kawabe K, Watanabe TX, Shiono K, Sokabe H (1979) Influence on blood pressure of renal isografts between spontaneously hypertensive and normotensive rats, utilizing the F1 hybrids. Jap Heart J 20:886
Klein TW, Gittes RF (1973) The three-kidney rat: renal isografts and renal counterbalance. J Urol 109:19
Maki T, Sakai A, Kountz SL (1975) The rat kidney as a model for the study of preservation methods. Transplantation 20:170
Muller-Suur R, Norlen B-J, Persson AEG (1980) Resetting of tubuloglomerular feedback in rat kidneys after unilateral nephrectomy. Kidney Int 18:48
Norlen BJ, Engberg A, Kallskog O, Wolgast M (1978) Nephron function of the transplanted rat kidney. Kidney Int 14:10
Norlen BJ, Muller-Suur R, Persson AEG (1978) Tubulo-glomerular feedback response and excretory characteristics of the transplanted rat kidney. Scand J Urol Nephrol 12:27
Oesterwitz H, Kaden J, Schneider W, Fritsch W (1988) Failure to prolong rat renal allograft survival time by photochemical donor kidney pretreatment during hypothermic pulsatile kidney preservation. Urol Res 16:53
Pegg DE (1981) Perfusion technology. In: Karow AM Jr, Pegg DE (eds) Organ preservation for transplantation. Dekker, New York, p 477
Provoost AP, DeKeijzer MH, Kort WJ, Wolff ED, Molenaar JC (1982) The glomerular filtration rate of isogeneically transplanted rat kidneys. Kidney Int 21:459
Schreiner GF, Flye W, Brunt E, Korber K, Lefkowith JB (1988) Essential fatty acid depletion of renal allografts and prevention of rejection. Science 240:1032
Silber SJ (1974) Chronic salt-loading of donor and recipient in renal transplantation. Surgery 75:573
Silber SJ, Crudup J (1974) The three-kidney rat model. Invest Urol 11:466
Silber S, Malvin RL (1974) Compensatory and obligatory renal growth in rats. Am J Physiol 226:114
Wallenstein S, Zucker CL, Fleiss JL (1980) Some statistical methods useful in Circulation Research. Circulation Res 47:1
Waynforth HB (1980) Experimental and surgical technique in the rat. Academic, New York, p 178
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Kline, R., Churchill, M., Churchill, P. et al. High osmolality-low pH flush solutions improve renal transplant function in rats. Urol Res 19, 81–86 (1991). https://doi.org/10.1007/BF00368181
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DOI: https://doi.org/10.1007/BF00368181