Summary
The cytotoxic effect of cis-diamminedichloro-platinum II (cisplatin, CDDP) on spermatogenesis in BALB/C mice, and possible protection of the testes by leuprolide acetate (D-Leu-6 LHRH(1–9)-ethylamide, TAP-144, leuprolide), a synthetic gonadotropin-releasing hormone analogue, were examined. Temporary interruption of the pituitary-gonadal axis by the analogue and amelioration of the gonadal toxicity of cisplatin by reducing the cell division rate in spermatogenesis were expected. The results showed cisplatin to have a cytotoxic effect on spermatogenic cells in BALB/C mice. The administration of leuprolide had no effect on testicular weight or histological findings in the mouse testes. Pretreatment and simultaneous administration of leuprolide did not reduce the damaging effect of cisplatin on the testes in mice. This is at variance with a previously published report.
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Brenner J, Vugrin D, Whitmore WF (1985) Effect of treatment on fertility and sexual function in males with metastatic nonseminomatous germ cell tumors of testis. Am J Clin Oncol 8:178
Calabresi P, Parks RE Jr (1985) Antiproliferative agents and drugs used for immunosuppression. In: Gilman AG, Goodman LS, Roll TW and Murad F (eds) The pharmacological basis of therapeutics, 7th edn. Macmillan, New York, p 1247
Coy DH, Coy EJ, Schally AV, Vilchez-Martinez J, Hirotsu Y, Arimura A (1974) Synthesis and biological properties of [D-Ala-6, des-Gly-NH-10]-LH-RH ethylamide, a peptide with greatly enhanced LH-and FSH-releasing activity. Biochem Biophys Res Commun 57:335
Cutis A, Lyons V, Fink G (1983) The human hypothalamic LH-RH precursor is the same size as that in rat and mouse hypothalamus. Biochem Biophys Res Commun 117:872
Fraley EE, Lange PH, Kennedy BJ (1983) Germ cell testicular cancer in adults. Medical progress. N Engl J Med 301:1420
Fujino M, Fukuda T, Shinagawa S, Kobayashi S, Yamazaki I, Seely JH, White WF, Rippel RH (1974) Synthetic analogue of luteinizing hormone releasing hormone (LH-RH) substituted in position 6 and 10. Biochem Biophys Res Commun 60:406
Glode LM, Robinson J, Gould SF (1981) Protection from cyclophosphamide induced testicular damage with an analogue of gonadotopin-releasing hormone. Lancet MAY 23:1132
Hunter MG, Sullivan MHF, Dix OJ, Alfred LF, Cooke BA (1982) Stimulation and inhibition by LHRH analogues of cultured rat Leydig cell function and lack of effect on mouse Leydig cells. Mol Cell Endocrinol 27:31
Jewett MAS, Jarvi K (1986) Infertility in patients with testicular cancer. In: Javadpour N (ed) Principles and management of testicular cancer. Thieme, New York, p 351
Johnsen SG (1970) Testicular biopsy score count — a method for registration of spermatogenesis in human testis: normal values and results in 355 hypogonadal males. Hormones 1:2
Kuhl H, Kaplan HG, Taubert HD (1976) Die Wirkung eines neuen Analogs des LH-RH, D-Ser(TBU)-EA-LHRH, auf die Gonadotropin-Freisetzung bei Männern. Dtsch Med Wochenschr 101:361
Matsuo M, Baba Y, Nair RM, Arimura A, Schally AV (1971) Structure of porcine LH- and FSH-releasing hormone. I. The proposed amino acid sequence. Biochem Biophys Res Common 43:1334
Meistrich ML, Hunter NR, Suzuki N, Trostle PK, Withers HR (1977) Gradual regeneration of mouse testicular stem cells after ionizing radiation. Radiat Res 74:349
Mian TA, Suzuki N, Glenn H, Haynie TP, Meistrich ML (1977) Radiation damage to mouse testis cells from [99mTc] pertechnetate. J Nucl Med 18:1116
Schally AV (1978) Aspects of hypothalamic regulation of the pituitary gland. Its implication for the control of reproductive processes. Science 202:18
Shilsky RL, Lewis BJ, Shrins RJ, Young RC (1980) Gonadal dysfunction in patients receiving chemotherapy for cancer. Ann Intern Med 93:109
Van Steenbrugge GJ, Romijn JC, de Jong FH, Schröder FH (1984) Unresponsiveness of the reproductive organs of the male mouse to treatment with a potent lutenizing hormone-releasing hormone agonist (ICI-118, 630). Urol Res 12:175
Sundaram K, Schmidt F, Thau RB, River J, Vale W, Bardin CW (1984) Species difference in the sensitivity to the antitesticular effects of [Ac-D-NAL(2), 4FD-Phe, D-Arg]-LHRH, a potent antagonist. Contraception 29:271
Thau RB, Limonta P, Schmidt F, Sundaram K (1985) Species differences in the sensitivity to GnRH analogs. J Steroid Biochem 23:811
Vickery BH (1983) Physiology and antifertility effects of LHRH agonistic analogs in male animals. In: Zatuchni GI, Shelton JD, Scierra J (eds) LHRH peptides as female and male contraceptives. Harper and Row, Philadelphia, p 134
Vickery BH (1985) Comparison of the potential utility of the LHRH agonists and antagonists for fertility control. J Steroid Biochem 23:779
Vilchez-Martinez JA, Coy DH, Arimura A, Coy EJ, Hirotsu Y, Schally AV (1974) Synthesis and biological properties of [Leu-6]-LH-RH and [D-Leu-6, des-Gly-NH]-LH-RH ethylamide. Biochem Biophys Res Commun 59:1226
Wang NG, Sundaram S, Pavlov S, River J, Vale W, Bardin CW (1983) Mice are insensitive to the antitesticular effects of lutenizing hormone-releasing hormone agonists. Endocrinology 112:331
Yen SSC (1983) Clinical application of gonadotropin-releasing hormone and gonadotropin-releasing hormone analogs. Fertil Steril 39:257
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Nonomura, M., Okada, K., Hida, S. et al. Does a gonadotropin-releasing hormone analogue prevent cisplatin-induced spermatogenic impairment?. Urol Res 19, 135–140 (1991). https://doi.org/10.1007/BF00368192
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DOI: https://doi.org/10.1007/BF00368192