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Effects of various HTK solution regimens on proteinuria after renal transplantation in dogs

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Abstract

Investigations were carried out by means of an autologous, heterotopic model for kidney transplantation applied to dogs. Duration of cold ischemia was 48 h. Four experimental groups were arranged. During the first 20 min following revitalization of the transplanted kidney, group 1 (HTK solution/80 cm perfusion height) showed a significant glomerular and tubular malfunction. In group 2 (HTK solution/120 cm perfusion height), only four urinary proteins with molecular weights of 25 kDa, 67 kDa, 100 kDa and >100 kDa were found. The excretion of higher molecular proteins receded over the 20-min period of observation. In both group 3 (HTK/aspartate solution) and group 4 (HTK/tryptophan solution) the quantity of excreted glomerular and tubular protein was well above that of group 2. As opposed to the “Tryptophan” group, a complete restoration of renal function was observed in the “Aspartate” group after 4 weeks. In general, the “standard” HTK protective solution delivered with 120 cm perfusion pressure gave the most favorable results, with the lowest levels of proteinuria and a satisfactory recovery of renal function after revitalization.

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References

  1. Alt JM, Jänig H, Schurek HJ, Stolte H (1979) Study of renal protein excretion in chronic pyelonephritis. Contrib Nephrol 16:37

    Google Scholar 

  2. Barsanti JA, Finco DR (1979) Protein concentration in urine of normal dogs. Am J Vet Res 40:1583

    Google Scholar 

  3. Bauer H, Nagel J, Wierer D, Brunner H, Franz HE (1985/1986) Evaluation of proteinuria by two-dimensional polyacrylamide gel electrophoresis after kidney transplantation. Uremia Invest 9:269

    Google Scholar 

  4. Besarab A, Ihle BU, Jarell B, Burke JF, Wesson LG (1983) Some effects of preservation and acute renal transplants. Am J Kidney Dis 2:456

    Google Scholar 

  5. Bloemendal H, Bout WS, Benedetti EL, Wisse JH (1965) On the subunits of alpha-crystalline. Exp Eye Res 4:319

    Google Scholar 

  6. Boesken WH, Schmidt M, Jontofsohn R, Heinze V (1975) Proteinuria as diagnostic marker after human kidney transplantation. EDTA 11:333

    Google Scholar 

  7. Braun WE, Merrill JP (1968) Proteins in normal and pathological urine. Transplantation 6:867

    Google Scholar 

  8. Butler EA, Flynn FV (1958) The proteinuria of renal tubular disorders. Lancet II:978

    Google Scholar 

  9. Davis BJ (1964) Disc electrophoresis II method and application to human serum proteins. Ann NY Acid Sci 121:404

    Google Scholar 

  10. Debray-Sachs M (1970) Study of proteinuria after kidney transplantation with long-term survival. In: Manuel Y, Revillard JP, Betuel H (eds) Proteins in normal and pathological urine. Karger, Basel, p 271

    Google Scholar 

  11. First MR, Vaidya PN, Maryniak RK, Weiss MA, Munda R, Fidler JP, Penn I, Alexander JW (1984) Proteinuria following transplantation. Transplantation 38:607

    Google Scholar 

  12. Frey BM, Frey FJ, Wegmüller E, Hodler J (1977) Proteinurie nach Nierentransplantation. Schweiz Med Wochenschr 107:1181

    Google Scholar 

  13. Heberer G, Land W (1979) Derzeitiger Stand der Organtransplantation. Schriftenr Bayer Landesärztekammer 49:7

    Google Scholar 

  14. Hemmingsen L, Jensen H, Skaarup P, Tetschke KI, Wallin AL (1976) The predictive value of specific protein clearances in detecting acute rejection episodes after renal transplantation. Clin Nephrol 5:163

    Google Scholar 

  15. Hulme B, Porter KA (1970) Macromolecular clearances following renal transplantation. In: Manuel Y, Revillard JP, Betuel H (eds) Proteins in normal and pathological urine. Karger, Basel, p 292

    Google Scholar 

  16. Kallerhoff M, Blech M, Kehrer G, Kleinert H, Langheinrich M, Siekmann W, Helmchen U, Bretschneider HJ (1987) Nierenfunktionsparameter nach Ischämiebelastung unter der Euro-Collins-Lösung oder unter der kardioplegischen Lösung HTK nach Bretschneider. Urologe A 26:96

    Google Scholar 

  17. Kallerhoff M, Blech M, Isemer FE, Kehrer G, Kleinert H, Langheinrich M, Helmchen U, Bretschneider HJ, (1988) Metabolic, energetic and structural changes in protected and unprotected kidneys at temperatures of 1°C and 25°C. Urol Res 16:57

    Google Scholar 

  18. Kallerhoff M, Blech M, Kehrer G, Langheinrich M, Helmchen U, Bretschneider HJ, Ringert RH (1989) Improvement in in situ renal protection against complete ischemia through the replacement of chloride by aspartate in the HTK solution of Bretschneider. Invest Urol 3:197

    Google Scholar 

  19. Krishna KS, Pandey AP, Kirubakaran MG, Kanagasabapathy AS (1987) Urinary protein/creatinine ratio as an indicator of allograft function following live related donor renal transplantation. Clin Chim Acta 163:51

    Google Scholar 

  20. Laterre EC, van Ypersele de Strihon C, Alexandre GPJ (1970) Proteinuria in human renal allograft with special reference to glomerular permeability in acute rejection crises. In: Manuel Y, Revillard JP, Betuel H (eds) Proteins in normal and pathological urine. Karger, Basel, p 300

    Google Scholar 

  21. Lindstedt E, Lindstedt G (1973) Tubular proteinuria early in nephronopthisis. Lancet II:1215

    Google Scholar 

  22. Ludwig A (1991) Experimentelle Untersuchungen am Hund zur Proteinurie, Reninaktivität und Blutdruckverhalten nach Nierentransplantation. Medical Dissertation, University of Göttingen

  23. Manuel Y, Poli S, Bernhardt JP, Revillard JP, Claudey D, Traeger J (1969/1970) Proteinuria in human renal allografts. Helv Med Acta 35:3

    Google Scholar 

  24. Neubüser G, Schenk H, Kielstein R, Lachein L (1983) Qualitative und quantitative Untersuchungen der Urinproteine von Patienten mit chronischer Niereninsuffizienz und nach Nierentransplantation. Z Med Diagn 24:88

    Google Scholar 

  25. Peterson PA, Evrin PE, Berggard L (1969) Differentiation of glomerular tubular and normal proteinuria determinations of urinary excretion of β2-Microglobulin, albumin, and total protein. J Clin Invest 48:1189

    Google Scholar 

  26. Pires MT, Da Dunha AS, Virella G, Simoes J (1975) Analytical characterization of urinary proteins by sodium dodecyl sulphate-polyacrylamide gel electrophoresis in renal disease. Nephron 14:361

    Google Scholar 

  27. Rapp W (1983) Renale Proteinausscheidung nach Nierentransplantation. Medical Dissertation, Medizinische Hochschule, Hannover

  28. Ravnskov U (1974) Proteinuria after human renal transplantation. Scand J Urol Nephrol 8:45

    Google Scholar 

  29. Raymond S (1962) A convenient apparatus for vertical gel electrophoresis. Clin Chem 8:455

    Google Scholar 

  30. Raymond S, Weintraub L (1959) Acrylamide gel as a supporting medium for zone electrophoresis. Science 130:711

    Google Scholar 

  31. Renner E, Edel H (1972) Immunoglobulinclearance bei der transplantierten Niere. In: Heintz R, Holzhüter H (eds) VIII Symposium der Gesellschaft der Nephrologie: Kongreßbericht der Technischen Hochschule, Aachen, p 181

  32. Revillard JP, Manuel Y, Betuel H, Fries D, Traeger J (1970) Proteinuria in human renal allografts-sequential studies. In: Manuel Y, Revillard JP, Betuel H (eds) Proteins in normal and pathological urine. Karger, Basel, p 309

    Google Scholar 

  33. Sethi K, First MR, Pesce AJ, Fidler JP, Pollak VE (1977) Proteinuria following renal transplantation. Nephron 18:49

    Google Scholar 

  34. Summers WK, Jamison RL (1971) The no reflow phenomenon in renal ischemia. Lab Invest 25:635

    Google Scholar 

  35. Veith FJ, Stenzel KH, Thompson DD, Rubin AL (1967) Urinary proteins from dog kidney transplants. Transplantation 5/6:1459

    Google Scholar 

  36. Weber MH (1988) Urinary protein analysis. J Chromatogr 429:315

    Google Scholar 

  37. Weber MH, Verwiebe R, Neuhoff V, Scheler F (1988) Analysis of proteinuria by micro-page electrophoresis. Contr Nephrol 68:179

    Google Scholar 

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, University of Göttingen, Robert-Koch-Strasse 40, D-37075, Göttingen, Germany

    A. Ludwig

  2. Department of General Surgery, St. Josef's Hospital, Solms Strasse 15, D-65189, Wiesbaden, Germany

    F. E. Isemer

  3. Department of Urology, University of Göttingen, Robert-Koch-Strasse 40, D-37075, Göttingen, Germany

    M. Kallerhoff

  4. Division of Nephrology, Department of Internal Medicine, Robert-Koch-Strasse 40, D-37075, Göttingen, Germany

    M. H. Weber

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  1. A. Ludwig
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  2. F. E. Isemer
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  3. M. Kallerhoff
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  4. M. H. Weber
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Ludwig, A., Isemer, F.E., Kallerhoff, M. et al. Effects of various HTK solution regimens on proteinuria after renal transplantation in dogs. Urol. Res. 23, 351–360 (1995). https://doi.org/10.1007/BF00300027

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  • DOI: https://doi.org/10.1007/BF00300027

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