Abstract
We have previously shown that the intramural motor nerves in the rat bladder can function in anoxic conditions. The present study aims to explore the distribution and activity of lactate dehydrogenase (LDH), the key enzyme for ATP generation in anoxia. The activity and isoform distribution pattern of LDH was studied in pelvic ganglia from male and female rats. A histochemical investigation showed that the LDH activity was intense in the ganglion cells, and weak in the other tissue components (nerve bundles, connective tissue). The male pelvic ganglion weighed 55% more than the female pelvic ganglion, the enzyme activity per unit ganglion weight was 60% higher and the total LDH activity was 155% higher. The isoform distribution was similar, with M4 being dominant isoform, followed by M3H. Infravesical outlet obstruction in the female rat induced a threefold increase in ganglion weight, and the total LDH activity increased twofold. In this hypertrophic female ganglion a decreased relative amount of M4, and an increased amount of MH3, was found. Diabetes in the male rat had no effect on ganglion weight or its contents and isoform distribution of LDH.
Similar content being viewed by others
References
Arner A, Malmquist U, Uvelius B (1990) Metabolism and force in hypertrophic smooth muscle from rat urinary bladder. Am J Physiol 258:C923
Arner A, Malmqvist U, Ősterman Å, Uvelius B (1993) Energy turnover and lactate dehydrogenase activity in detrusor smooth muscle from rats with streptozotocin-induced diabetes. Acta Physiol Scand 147:375
Baljet B, Drukker J (1980) The extrinsic innervation of the pelvic organs in the female rat. Acta Anat (Basel) 107:241
Chang AY, Noble RE, Wyse BM (1977) Streptozotocin-induced diabetes in the chinese hamster. Diabetologia 13:595
Gabella G, Uvelius B (1990) Urimary bladder of the rat: fine structure of normal and hypertrophic musculature. Cell Tissue Res 262:67
Gabella G, Berggren T, Uvelius B (1992) Hypertrophy and reversal of hypertrophy in rat pelvic ganglion neurons. J Neurocytol 21:649
Greene DA, Winegrad AI (1979) In vitro studies of the substrates for energy production and the effects of insulin on glucose utilization in the neural components of peripheral nerve. Diabetes 28:878
Greene DA, Winegrad AI (1981) Effects of acute experimental diabetes on composite energy metabolism in peripheral nerve axons and Schwann cells. Diabetes 30:967
Greenwood D, Coggeshall RE, Holsebosch CE (1985) Sexual dimorphism in the number of neurons in the pelvic ganglia of adult rats. Brain Res 340:160
den Hertog A, Greengard P, Ritchie JM (1969) On the metabolic basis of nervous activity. J Physiol (Lond) 204:511
Jacobsen NO (1969) The histochemical localization of lactic dehydrogenase isoenzymes in the rat nephron by means of an improved polyvinyl alcohol method. Histochemic 20:250
Keast JR, Booth AM, de Groat WC (1989) Distribution of neurons in the major pelvic ganglion of the rat which supply the bladder, colon or penis. Cell Tissue Res 256:105
Langworthy OT (1965) Innervation of the pelvic organs of the rat. Invest Urol 2:491
Low PA, Tuck RR, Takeuchi M (1987) Nerve microenvironment in diabetic neuropathy. In: Dyck PJ, Thomas PK, Asbury AK, Winegrad AI, Porte D Jr (eds) Diabetic neuropathy. Saunders, Philadelphia, p 266
Malmgren A, Sjögren C, Uvelius B, Mattiasson A, Andersson K-E, Andersson PO (1987) Cystometrical evaluation of bladder instability in rats with infravesical outflow obstruction. J Urol 137:1291
Malmqvist U, Arner A, Uvelius B (1991) Lactate dehydrogenase activity and isoform distribution in normal and hypertrophic smooth muscle tissue from the rat. Pfluegers Arch 419:230
Mattiasson A, Uvelius B (1982) Changes in contractile properties in hypertrophic rat urinary bladder. J Urol 128:1340
Paul RJ (1980) Chemical energetics of vascular smooth muscle. In: The cardiovascular system. Vascular smooth muscle. American Physiol Soc. Bethesda, p 237 (Handbook of Physiology, Vol 2)
Paul RJ, Bauer M, Peace W (1979) Vascular smooth muscle: aerobic glycolysis linked to sodium and potassium transport processes. Science 206:1414
Polyanska M, Arner A, Malmqvist U, Uvelius B (1993) Lactate dehydrogenase activity and isoform distribution in the rat urinary bladder: effects of outlet obstruction. J Urol 150:543
Siesjsö BK (1979) Brain energy metabolism. Wiley, New York
Steers WD, Ciambotti J, Erdman S, de Groat WC (1990) Morphological plasticity in efferent pathways to the urinary bladder of the rat following urethral obstruction. J Neurosci 10:1943
Steers WD, Mackway-Gerardi AM, Ciambotti J, de Groat WC (1994) Alterations in neuronal pathways to the urinary bladder of the rat in responses to streptozotocin-induced diabetes. J Auton Nerv Syst 47:83
Stewart MA, Passoneau JV, Lowry OH (1965) Substrate changes in peripheral nerve during ischaemia and Wallerian degeneration. J Neurochem 12:719
Winegrad AI, Simmons DA (1987) Energy metabolism in peripheral nerve. In: Dyck PJ, Thomas PK, Asbury AK, Winegrad AI, Porte D Jr (eds) Diabetic neuropathy. Saunders, Philadelphia, p 279
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Berggren, T., Arner, A. & Uvelius, B. Lactate dehydrogenase activity and isoform distribution in the rat pelvic ganglion: effects of diabetes and bladder outlet obstruction. Urol. Res. 23, 395–399 (1995). https://doi.org/10.1007/BF00698742
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00698742