Summary
The possibility of using hydrogen-peroxide as an oxygen source in immobilized whole cell systems was investigated.
The conversion of glycerol to dihydroxyacetone performed by Ca-alginate immobilized Gluconobacter oxydans was used as a model system. The influence of basic parameters, such as pH, temperature and concentration of hydrogen-peroxide, on the conversion are reported. Continuous production using a packed bed could be substantially improved by using hydrogen-peroxide as a source of oxygen. The productivity was increased almost twenty-fold in the reactor. However, hydrogen-peroxide also caused deactivation of the oxidizing of the cells.
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Holst, O., Enfors, SO. & Mattiasson, B. Oxygenation of immobilized cells using hydrogen-peroxide; a model study of Gluconobacter oxydans converting glycerol to dihydroxyacetone. European J. Appl. Microbiol. Biotechnol. 14, 64–68 (1982). https://doi.org/10.1007/BF00498004
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DOI: https://doi.org/10.1007/BF00498004