Summary
Streptomyces rimosus cells were immobilized with urethane prepolymers and used in the production of oxytetracycline. Based on the criteria for oxytetracycline productivity, cell growth in gels, cell leakage from gels and mechanical strength of gel, a hydrophilic prepolymer, PU-1, the main chain of which was polyethylene glycol (molecular weight, approximately 1500) was employed as gel material among 11 kinds of urethane prepolymers. Use of glucose-free medium for cultivation of PU-1-entrapped cells increased the production rate of oxytetracycline and minimized cell leakage from the gels. When the gel-entrapped cells lost activity, treatment of the cell-entrapping gels with saline or 70% ethanol resulted in recovery of the oxytetracycline productivity. Continuous oxytetracycline fermentation using PU-1-entrapped growing cells was successfully achieved in air-bubbled reactor for at least 35 days with reactivation of the cells.
Similar content being viewed by others
References
Abbott BJ (1976) Preparation of pharmaceutical compounds by immobilized enzymes and cells. Adv Appl Microbiol 20:203–257
Arcuri EJ, Nichols JR, Brix TS, Santamarina VG, Buckland BC, Drew SW (1983) Thienamycin production by immobilized cells of Streptomyces cattleya in a bubble column. Biotechnol Bioeng 25:2399–2411
Berk D, Behie LA, Jones A, Lesser BH, Gaucher GM (1984a) The production of the antibiotic patulin in a three phase fluidized bed reactor: I. Effect of medium composition. Can J Chem Eng 62:112–119
Berk D, Behie LA, Jones A, Lesser BH, Gaucher GM (1984b) The production of the antibiotic patulin in a three phase fluidized bed reactor: II. Longevity of the biocatalyst. Can J Chem Eng 62:120–124
Chibata I, Tosa T (1977) Transformation of organic compounds by immobilized microbial cells. Adv Appl Microbiol 22:1–27
Deo YM, Gaucher GM (1984) Semicontinuous and continuous production of penicillin-G by Penicillium chrysogenum cells immobilized in k-carrageenan beads. Biotechnol Bioeng 26:285–295
Deo YM, Gaucher GM (1985) Effect of nitrogen supplementation on the longevity of antibiotic production by immobilized cells of Penicillium urticae. Appl Microbiol Biotechnol 21:220–227
Fukui S, Sonomoto K, Itoh N, Tanaka A (1980) Several novel methods for immobilization of enzymes, microbial cells and organelles. Biochimie 62:381–386
Fukui S, Tanaka A (1982) Immobilized microbial cells. Ann Rev Microbiol 36:145–172
Fukui S, Tanaka A (1984) Application of biocatalysts immobilized by prepolymer methods. Adv Biochem Eng Biotechnol 29:1–33
Kokubu T, Karube I, Suzuki S (1981) Protease production by immobilized mycelia of Streptomyces fradiae. Biotechnol Bioeng 23:29–39
Mazumder TK, Sonomoto K, Tanaka A, Fukui S (1985) Sequential conversion of cortexolone to prednisolone by immobilized mycelia of Curvularia lunata and immobilized cells of Arthrobacter simplex. Appl Microbiol Biotechnol 21:154–161
Morikawa Y, Karube I, Suzuki S (1980a) Continuous production of bacitracin by immobilized living whole cells of Bacillus sp. Biotechnol Bioeng 22:1015–1023
Morikawa Y, Karube I, Suzuki S (1980b) Enhancement of penicillin acylase activity by cultivating immobilized Kluyvera citrophila. Eur J Appl Microbiol Biotechnol 10:23–30
Nojima S (1983) The development of alcohol fermentation by immobilized living cells. Chem Econ Eng Rev 15 (4):17–22
Sonomoto K, Tanaka A, Omata T, Yamane T, Fukui S (1979) Application of photo-crosslinkable resin prepolymers to entrap microbial cells. Effects of increased cell-entrapping gel hydrophobicity on the hydrocortisone Δ1-dehydrogenation. Eur J Appl Microbiol Biotechnol 6:325–334
Sonomoto K, Jin IN, Tanaka A, Fukui S (1980) Application of urethane prepolymers to immobilization of biocatalysts: Δ1-Dehydrogenation of hydrocortisone by Arthrobacter simplex cells entrapped with urethane prepolymers. Agric Biol Chem 44:1119–1126
Sonomoto K, Hoq MM, Tanaka A, Fukui S (1981) Growth of Curvularia lunata spores into mycelial form within various gels, and steroid 11β-hydroxylation by the entrapped mycelia. J Ferment Technol 59:465–469
Sonomoto K, Nomura K, Tanaka A, Fukui S (1982) 11α-Hydroxylation of progesterone by gel-entrapped living Rhizopus stolonifer mycelia. Eur J Appl Microbiol Biotechnol 16:57–62
Sonomoto K, Hoq MM, Tanaka A, Fukui S (1983a) 11β-Hydroxylation of cortexolone (Reichstein Compound S) to hydrocortisone by Curvularia lunata entrapped in photocross-linked resin gels. Appl Environ Microbiol 45:436–443
Sonomoto K, Usui N, Tanaka A, Fukui S (1983b) 9α-Hydroxylation of 4-androstene-1, 17-dione by gel-entrapped Corynebacterium sp. cells. Eur J Appl Microbiol Biotechnol 17:203–210
Tanaka A, Yasuhara S, Osumi M, Fukui S (1977) Immobilization of yeast microbodies by inclusion with photo-cross-linkable resins. Eur J Biochem 80:193–197
Tanaka A, Sonomoto K, Hoq MM, Usui N, Nomura K, Fukui S (1982) Hydroxylation of steroids by immobilized microbial cells. Enzyme Engineering 6:131–133
Veelken M, Pape H (1982) Production of tylosin and nikkomycin by immobilized Streptomyces cells. Eur J Appl Microbiol Biotechnol 15:206–210
Veelken M, Pape H (1984) Production of nikkomycin by immobilized Streptomyces cells — Physiological properties. Appl Microbiol Biotechnol 19:146–152
Yongsmith B, Sonomoto K, Tanaka A, Fukui S (1982) Production of vitamin B12 by immobilized cells of a propionic acid bacterium. Eur J Appl Microbiol Biotechnol 16:70–74
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ogaki, M., Sonomoto, K., Nakajima, H. et al. Continuous production of oxytetracycline by immobilized growing Streptomyces rimosus cells. Appl Microbiol Biotechnol 24, 6–11 (1986). https://doi.org/10.1007/BF00266277
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00266277