Summary
A novel microorganism producing a maltopentaose-forming enzyme was screened from a soil sample. The enzyme produced by the bacteria formed maltopentaose from starch during the first stage of the reaction. The bacterium was characterized asPseudomonas sp. (KO-8940) on the basis of its morphological, physiological and biological properties. Culture conditions for enzyme production were investigated and established. The medium was composed of beef extract 0.8%, ammonium sulphate 1.0% and maltose 0.8% in tap water. Optimum conditions for bacterial growth were: initial pH 8.0, temperature 45°C, and 3 days cultivation with a rotary shaker (200 rpm). The broth supernatant obtained contained the maltopentaose-forming crude enzyme, with which 40% of starch was converted to maltopentaose.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Berlyne GM, Booth EM, Brewis RAL, Mallick NP, Simons PJ (1969) A soluble glucose polymer for use in renal failure and calorie-deprivation states. Lancet 1:689–692
Kainuma K, French D (1969) Action of pancreatic amylase on starch oligosaccharides containing single glucose side chains. FEBS Lett 5:257–261
Kainuma K, Kobayashi S, Ito T, Suzuki S (1972) Isolation and action pattern of maltohexaose producing amylase fromAerobacter aerogenes. FEBS Lett 26:281–285
Kainuma K, Wako K, Nogami A, Suzuki S (1973) Automated assays of carbohydrase activity by Technicon autoanalyzer. J Jap Soc Starch Sci 20:112–119
Kennedy JF, White CA (1979) Characteristics of α-amylase K, a novel amylase from a strain ofBacillus subtilis. Stärke 31:93–99
Robyt JF, French D (1963) Action pattern and specificity of an amylase fromBacillus subtilis. Arch Biochem Biophys 100:451–467
Robyt JF, Ackerman RJ (1971) Isolation, purification and characterization of a maltotetraose producing amylase fromPseudomonas stutzeri. Arch Biochem Biophys 145:105–114
Saito N (1973) A thermophilic extracellular α-amylase fromBacillus licheniformis. Arch Biochem Biophys 155:290–298
Saito N (1982) Evaluation of maltopentaose as a substrate for α-amylase determination in serum. J Jap Soc Starch Sci 29:153–160
Shimidu U (1976) “Shokuhin Biseibutsugaku”, ed. by, K. Aiso, Ishiyaku Press Inc., Tokyo, p 12
Shinke R, Kunimi Y, Nishira H (1975) Isolation and characterization of β-amylase producing microorganisms; Production and some properties of β-amylases ofBacillus sp. BQ 10. J Ferment Tecynol 53:687–692; 693–697
Shinke R, Nishira H (1981) β-Amylase production by microorganisms. J Jap Soc Starch Sci 28:67–71
Somogyi M (1952) Notes on sugar determination. J Biol Chem 195:19–23
Takasaki Y (1982) Maltohexaose-producing amylase fromBacillus sp. J Jap Soc Starch Sci 29:145–152
Takasaki Y (1983) An amylase producing maltotetraose and maltopentaose fromBacillus circulans. Agric Biol Chem 47:2193–2199
Takasaki Y (1985) An amylase producing maltotriose fromBacillus subtilis. Agric Biol Chem 49:1091–1097
Taniguchi H, Jae CM, Yoshigi N, Maruyama Y (1983) Purification of Bacillus circulans F-2 amylase and its general properties. Agric Biol Chem 47:511–519
Yoshigi N, Chikano T, Kamimura M (1985) Characterization of a maltopentaose-producing bacterium and its cultural conditions. Agric Biol Chem 49:2379–2384
Wako K, Takahashi C, Hashimoto S, Kanaeda J (1978) Studies on maltotriose- and maltose-forming amylases fromStreptomyces. J Jap Soc Starch Sci 25:155–161
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Okemoto, H., Kobayashi, S., Momma, M. et al. Isolation and cultivation of a novel microorganism producing a maltopentaose-forming enzyme. Appl Microbiol Biotechnol 25, 137–142 (1986). https://doi.org/10.1007/BF00938937
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00938937