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On a viologen-dependent pyridine nucleotide oxidoreductase from a thermophilic Bacillus

  • Applied Microbiology
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Summary

From a thermophilic bacillus a viologen dependent pyridine nucleotide oxidoreductase has been purified and partially characterized. Its apparent molecular weight is about 120000 consisting of two subunits of equal or very similar molecular weight. Per molecular weight of 120000 the enzyme contains 4 FAD. FMN or labile sulfur could not be detected. The physiological role of the enzyme is not clear. It reduces NAD as well as NADP at the expense of reduced methylviologen. The reduced pyridine nucleotides can be reoxidized with carbamoylmethylviologen. The seven determined K m- and six K i-values show that the enzyme is suitable for the regeneration of NADH, NADPH, NAD and NADP. The stability in presence of oxidized and reduced methylviologen at 35°C or 60°C is satisfying for preparative work.

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Abbreviations

MV:

Methylviologen species

MV2+ :

1,1′-dimethyl-4,4′-bipyridinium dication=methylviologen oxidized

MV- :

methylviologen cation radical=methylviologen reduced

CAV:

carbamoylmethylviologen species

VDPNOR:

Viologen dependent pyridine nucleotide oxidoreductase

DSM:

Deutsche Sammlung für Mikroorganismen

Tris:

tris(hydroxymethyl)aminomethane

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Authors and Affiliations

  1. Institute for Organic Chemistry, Technical University Munich, D-8046, Garching, Germany

    Sinichi Nagata, Richard Feicht, Winfried Bette, Helmut Günther & Helmut Simon

Authors
  1. Sinichi Nagata
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  2. Richard Feicht
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  3. Winfried Bette
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  4. Helmut Günther
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  5. Helmut Simon
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Nagata, S., Feicht, R., Bette, W. et al. On a viologen-dependent pyridine nucleotide oxidoreductase from a thermophilic Bacillus. Appl Microbiol Biotechnol 26, 263–267 (1987). https://doi.org/10.1007/BF00286321

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  • DOI: https://doi.org/10.1007/BF00286321

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