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The involvement of an enantioselective transaminase in the metabolism of D-3- and D-4-hydroxyphenylglycine inPseudomonas putida LW-4

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Summary

Pseudomonas putida LW-4, isolated on D-phenylglycine as sole carbon and energy source, was also able to grow on D-3-and D-4-hydroxyphenylglycine. Both D-3-and D-4-hydroxyphenylglycine were initially converted to the corresponding hydroxyphenylglyoxylates by means of an enantioselective transaminase. Subsequently, the hydroxyphenylglyoxylates were decarboxylated and then oxidized to 3-and 4-hydroxybenzoate, respectively. These latter compounds in turn were oxidized by NADPH-dependent hydroxylases to protocatechuate, which was further oxidized via an intradiol cleavage. Preliminary experiments with cell extracts in which the 4-hydroxyphenylglyoxylate decarboxylase was partially removed by an ammonium sulfate fractionation showed that D-4-hydroxyphenylglyoxycine could be formed from 4-hydroxyphenylglyoxylate by the enantioselective transaminase.

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Author notes

  1. W. J. J. van den Tweel

    Present address: DSM Research Bio-organic Chemistry Section, P.O. Box 18, 6160 MD, Geleen, The Netherlands

Authors and Affiliations

  1. Division of Industrial Microbiology, Department of Food Science, Agricultural University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands

    W. J. J. van den Tweel, J. P. Smits & J. A. M. de Bont

  2. Andeno B.V., Post Office Box 81, 5900 AB, Venlo, The Netherlands

    R. L. H. P. Ogg

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  1. W. J. J. van den Tweel
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  2. J. P. Smits
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  3. R. L. H. P. Ogg
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  4. J. A. M. de Bont
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van den Tweel, W.J.J., Smits, J.P., Ogg, R.L.H.P. et al. The involvement of an enantioselective transaminase in the metabolism of D-3- and D-4-hydroxyphenylglycine inPseudomonas putida LW-4. Appl Microbiol Biotechnol 29, 224–230 (1988). https://doi.org/10.1007/BF01982906

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  • DOI: https://doi.org/10.1007/BF01982906

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