Skip to main content
SpringerLink
Log in

Identification of a starch degrading anaerobic thermophile producing thermostable α-amylase and pullulanase

  • Applied Microbiology
  • Short Contribution
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Summary

A thermophilic bacterium which was recently isolated and designated Clostridium sp. EM1 has been now identified and characterized. On the basis of its physiological characeristics and DNA-DNA homology data, the strain was designated as Clostridium thermosulfurogenes (DSM 3896). The guanine-plus-cytosine (G+C) content (mol%) of the DNA was determined to be 37%.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Antranikian G, Herzberg C, Gottschalk G (1987) Production of thermostable α-amylase, pullulanase, and α-glucosidase in continuous culture by a new clostridium isolate. Appl Environ Microbiol 53:1668–1673

    Google Scholar 

  • Aunstrup K (1979) Production, isolation, and economics of extracellular enzymes. Appl Biochem Bioeng 2:27–69

    Google Scholar 

  • De Ley J (1970) Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J Bacteriol 101:738–754

    Google Scholar 

  • Fogarty WM, Kelly CT (1979) Starch degrading enzymes of microbial origin. Prog Ind Microbiol 15:87–150

    Google Scholar 

  • Holdeman LV, Cato EP, Moore WEC (1977) Anaerobe laboratory manuel, 4th edn. Virginia Polytechnic Institute and State University, Blacksburg, Va., USA

    Google Scholar 

  • Madi E, Antranikian G, Ohmiya K, Gottschalk G (1987) Thermostable amylolytic enzymes from a new clostridium isolate. Appl Environ Microbiol 53:1661–1667

    Google Scholar 

  • Marmur J (1961) A procedure for the isolation of DNA from microorganisms. J Mol Biol 3:208–218

    Google Scholar 

  • Marmur J, Doty P (1962) Determination of the base composition of DNA from its thermal denaturation temperature. J Mol Biol 5:109–118

    Google Scholar 

  • Priest FG (1984) Extracellular enzymes. Aspects of microbiology, vol 9. Van Nostrand Reinhold, UK

    Google Scholar 

  • Schink B, Zeikus JG (1983) Clostridium thermosulfurogenes sp. nov., a new thermophile that produces elemental sulfur from thiosulfate. J Gen Microbiol 129:1149–1158

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Mikrobiologie der Georg-August Universität Göttingen, Grisebachstrasse 8, D-3400, Göttingen, Federal Republic of Germany

    E. Madi & G. Antranikian

Authors
  1. E. Madi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Antranikian
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Madi, E., Antranikian, G. Identification of a starch degrading anaerobic thermophile producing thermostable α-amylase and pullulanase. Appl Microbiol Biotechnol 30, 422–425 (1989). https://doi.org/10.1007/BF00296634

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00296634

Keywords

Search

Navigation