Skip to main content
SpringerLink
Log in

Interrelationship between pH-dependent uptake of bromophenol blue by intact Saccharomyces cerevisiae cells and their viability after dehydration

  • Applied Microbiology
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Summary

An inverse linear relationship (n=16; r=0.929; 1-P=0.001) was established between the viability of dehydrated-rehydrated yeasts and the uptake of a weak acid, bromophenol blue, by samples of intact cells. The dependence indicates the fact that yeasts that are more resistant to dehydration have lower intracellular pH values, and hence can be used to predict the viability of yeasts to be dried.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Beker MJ, Damberg BE, Rapoport AI (1981) Anabiosis of microorganisms (in Russian). Zinatne, Riga

    Google Scholar 

  • Borst-Pauwels GWFH (1981) Ion transport in yeast. Biochim Biophys Acta 650:88–127

    Google Scholar 

  • Gadd GM, Chalmers K, Reed RH (1987) The role of trehalose in dehydration resistance of Saccharomyces cerevisiae. FEMS Microbiol Lett 48:249–254

    Google Scholar 

  • Gillies PR (1981) Intracellular pH and control of growth in eucaryotic cells. In: Kameron IL, Pool ThB (eds) The transformed cell. Academic Press, London, pp 317–361

    Google Scholar 

  • Kleiner D (1981) The transport of NH3 and NH +4 across biological membranes. Biochem Biophys Acta 639:41–52

    Google Scholar 

  • Kotyk A (1963) Intracellular pH of baker's yeast. Folia Microbiol 8:27–31

    Google Scholar 

  • Kotyk A, Kleinzeller A (1958) Movement of sodium and cell volume changes in a sodium-rich yeast. J Gen Microbiol 20:197–212

    Google Scholar 

  • Serrano R (1988) Structure and function of proton translocating ATPase in plasma membranes of plants and fungi. Biochim Biophys Acta 947:1–28

    Google Scholar 

  • Slavik I, Kotyk A (1984) Intracellular pH distribution and transmembrane pH profile of yeast cells. Biochim Biophys Acta 766:679–684

    Google Scholar 

  • Zikmanis PB, Auzina LP, Auzane SI, Beker MJ (1982) Relationship between the fatty acid composition of lipids and the viability of dried yeast Saccharomyces cerevisiae. Eur J Appl Microbiol Biotechnol 15:100–103

    Google Scholar 

  • Zikmanis PB, Auzane SI, Kruce RV, Auzina LP, Beker MJ (1983) Interrelationships between the fatty acid composition and metabolic pathways upon dehydration-rehydration of yeast Saccharomyces cerevisiae. Eur J Appl Microbiol Biotechnol 18:298–302

    Google Scholar 

  • Zikmanis PB, Laivenieks MG, Auzina LP, Kulaev IS, Beker MJ (1985) Interrelation between high-molecular polyphosphate content, trehalose and viability of dehydrated yeast Saccharomyces cerevisiae population (in Russian). Mikrobiologiya 54:406–409

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. August Kirchenstein Institute of Microbiology, Latvian SSR Academy of Sciences, I, A. Kirchenstein Street, 226067, Riga, Latvian SSR, USSR

    P. B. Zikmanis, R. V. Kruce, I. E. Mackare, L. P. Auzina & M. J. Bekers

Authors
  1. P. B. Zikmanis
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. V. Kruce
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I. E. Mackare
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. P. Auzina
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. J. Bekers
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zikmanis, P.B., Kruce, R.V., Mackare, I.E. et al. Interrelationship between pH-dependent uptake of bromophenol blue by intact Saccharomyces cerevisiae cells and their viability after dehydration. Appl Microbiol Biotechnol 31, 191–193 (1989). https://doi.org/10.1007/BF00262461

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00262461

Keywords

Search

Navigation