Summary
Mutants of Candida maltosa were isolated that lacked saccharopine reductase (lys9) and saccharopine dehydrogenase (lys1) and were able to accumulate α-aminoadipate-δ-semialdehyde (AASA) in the cell and excrete it into the culture medium. The effects of incubation time, lysine concentration, and carbon and nitrogen sources on AASA production were examined. In the presence of 15 g glucose/1, 1.25 g NH4H2PO4/l and 50 mg l-lysine/l in a minimal salt medium C. maltosa G285 (lys1) produced about 80–90 mg AASA/l during 48 h of growth. A simple and rapid procedure to isolate AASA from the medium using Dowex 50X4 is described.
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Schmidt, H., Bode, R., Samsonova, I.A. et al. Production of α-aminoadipate-δ-semialdehyde by a mutant from Candida maltosa . Appl Microbiol Biotechnol 31, 463–466 (1989). https://doi.org/10.1007/BF00270777
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DOI: https://doi.org/10.1007/BF00270777