Summary
Erwinia chrysanthemi cells were used to study the possibility of producing bacterial enzymes in a bioreactor coupled with a membrane filtration unit. Continuous fermentations with total cell recycle failed to give good production of pectate lyase (PL). Enzymatic, mechanical and physico-chemical damages were involved in this phenomenon. With a sequential recycle mode, we obtained productivity of 1.5 units·h−1·1−1 with a high PL concentration. Protease accumulation occurred when the bioreactor was coupled to a filtration unit. Moreover we have observed no loss of activity due to high shear stress caused by pumping.
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Denis, S., Boyaval, P. Microbial enzyme production in a membrane bioreactor. Appl Microbiol Biotechnol 34, 608–612 (1991). https://doi.org/10.1007/BF00167908
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DOI: https://doi.org/10.1007/BF00167908