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Physiology of polyol formation by free and immobilized cells of the osmotolerant yeast Pichia farinosa

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Summary

Some physiological data of cells of Pichia farinosa immobilized on sintered glass Raschig rings were compared with data from free cells. Glucose consumption and productivity of total polyols (arabitol, glycerol and erythritol) showed a simultaneous “inter-lag” phase. Enzymes that catalyse steps of the pentosephosphate pathway (glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, transaldolase and polyol dehydrogenase) showed a distinct increase after transfer of the cells into production medium. The activity of glycerol-3-phosphate dehydrogenase was generally low. Only alcohol dehydrogenase presented the inter-lag phase mentioned above.

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Authors and Affiliations

  1. Institut für Mikrobiologie, Universität Münster, Corrensstraasse 3, W-4400, Münster, Federal Republic of Germany

    Ute Höötmann, Bernward Bisping & Hans-Jürgen Rehm

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  1. Ute Höötmann
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  2. Bernward Bisping
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  3. Hans-Jürgen Rehm
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Höötmann, U., Bisping, B. & Rehm, HJ. Physiology of polyol formation by free and immobilized cells of the osmotolerant yeast Pichia farinosa . Appl Microbiol Biotechnol 35, 258–263 (1991). https://doi.org/10.1007/BF00184698

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  • DOI: https://doi.org/10.1007/BF00184698

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