Summary
We have developed an improved artificial seed system by using a hot-water extract from a marine cyanobacterium, Synechococcus sp. NKBG 042902. Carrot somatic embryos (Daucus carota L.) were divided into two size categories (> 800 μm and 425–800 μm). High frequency germination (91%) was obtained using the large somatic embryos encapsulated in calcium alginate gel containing 400 mg 1−1 of extract. This compares to 35% without addition of the extract. A non-dialysate fraction of the extract showed strong germination-promoting activity compared with a dialysate fraction. The germination frequency of artificial seeds containing 100 mg 1−1 of non-dialysate fraction was more than 90%. Almost all germinating artificial seeds developed into plantlets within 4 days. We also achieved high frequency germination (60%) of artificial seeds encapsulating small somatic embryos (425–800 μm) that contained 100 mg 1−1 of non-dialysate (control 9%). Although the small somatic embryos showed a lower germination frequency than the large embryos, the plantlet development process in these seeds was far more vigorous. Such a high germination frequency has not previously been reported for a carrot artificial seed system.
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References
Fujii JA, Slade DT, Redenbaugh K (1987) Artificial seeds for plant propagation. Tibtech 5:335–339
Fujimura T, Komamine A (1975) Effects of various growth regulators on the embryogenesis in a carrot cell suspension culture. Plant Sci Lett 5:359–364
Gray DJ (1987) Synthetic seed technology for the mass cloning of crop plants: problems and perspectives. Hortic Science 22:795–814
Halperin W, Wetherell DF (1964) Adventive embryony in tissue cultures of the wild carrot, Daucus carota. Am J Bot 51:274–283
Herdman M (1988) Cellular differentiation: akinetes. In: Packer L, Glazer AN (eds) Cyanobacteria, methods in enzymology, vol 167. Academic Press, San Diego, pp 222–231
Herdman M, Rippka R (1988) Cellular differentiation: hormogonia and baeocytes. In: Packer L, Glazer AN (eds) Cyanobacteria, methods in enzymology, vol 167. Academic Press, San Diego, pp 232–244
Hirano M, Mori H, Miura Y, Nakamura N, Matsunga T (1990) γ-Linolenic acid production by microalgae. Appl Biochem Biotechnol 24/5:183–191
Kaimori N (1988) A basic study of the development of somatic seeds. Regeneration from dried embryos of carrot. Bull Inst Agric Res Tohoku Univ 39:21–27
Kamada H, Harada H (1979) Studies on the organogenesis in carrot tissue cultures. I. Effects of growth regulators on somatic embryogenesis and root formation. Z Pflanzenphysiol 91:255–266
Kamada H, Kobayashi K, Kiyosue T, Harada H (1989) Stress induced somatic embryogenesis in carrot and its application to synthetic seed production. In Vitro Cell Dev Biol 25:1163–1166
Kitto CL, Janick J (1985) Hardening treatments increase survival of synthetically-coated asexual embryos of carrot. J Amer Soc Hortic Sci 110:285–286
Matsunaga T, Izumida H (1984) Seawater-based methane production from blue-green algae biomass by marine bacteria co-culture. Biotechnol Bioeng Symp 14:407–418
Matsunaga T, Nakamura N, Tsuzaki N, Takeda H (1988) Selective production of glutamate by an immobilized marine blue-green alga, Synechococcus sp. Appl Microbiol Biotechnol 28:373–376
Matsunaga T, Takeyama H, Nakamura N (1990) Characterization of cryptic plasmids from marine cyanobacteria and construction of a hybrid plasmid potentially capable of transformation of marine cyanobacterium, Synechococcus sp., and its transformation. Appl Biochem Biotechnol 24/5:151–160
Metting B, Pyne J (1986) Biologically active compounds from microalgae. Enzyme Microb Technol 8:386–394
Murashige T, Skoog FS (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–479
Redenbaugh K, Paasch BD, Nichol JW (1986) Somatic seeds: encapsulation of asexual plant embryos. Bio/Technology 4:781–797
Rippka R, Dereulles J, Waterbury JB, Herdman M, Stanier RY (1979) General assignment, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111:1–61
Smith RL, Van Baalen C, Tabita FR (1988) Isolation of metabolically active heterocysts from cyanobacteria. In: Packer L, Glazer AN (eds) Cyanobacteria, methods in enzymology, vol 167. Academic Press, San Diego, pp 490–495
Steward FC, Mapes MO, Mears K (1958) Growth and organized development of cultured cells, II. Organization in cultures grown from freely suspended cells. Am J Bot 45:705–708
Stuart DA, Redenbaugh K (1987) Use of somatic embryogenesis for the regeneration of plants. In: LeBaron HM, Mumma RO, Honeycutt RC, Duesing JH (eds) Biotechnology in agricultural chemistry. Am Chem Soc Symp Ser., vol 334. American Chemical Society, Washington, DC, pp 87–96
Stuart DA, Nelsen J, Strickland SG (1985) Factors affecting developmental processes in alfalfa cell cultures. In: Henke RR, Hughes KW, Constantin MP (eds) Tissue culture forestry and agriculture. Plenum Press, New York, pp 59–73
Wake H, Umetsu H, Ozeki Y, Matsunaga T (1989) Maturation and germination promoting factors of artificial seeds isolated from marine cyanobacteria. In: Miyache S, Karube I, Ishida Y (eds) Current topics in marine biotechnology. Japanese Society for Marine Biotechnology, Tokyo, pp 177–180
Wake H, Umetsu H, Ozeki Y, Shimomura K, Matsunaga T (1991) Extracts of marine cyanobacteria stimulated somatic embryogenesis of Daucus carota L. Plant Cell Rep 9:655–658
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Wake, H., Akasaka, A., Umetsu, H. et al. Enhanced germination of artificial seeds by marine cynobacterial extract. Appl Microbiol Biotechnol 36, 684–688 (1992). https://doi.org/10.1007/BF00183250
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DOI: https://doi.org/10.1007/BF00183250