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A genetically modified solvent-tolerant bacterium for optimized production of a toxic fine chemical

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Abstract

The aim of the study was to investigate whether toxic fine chemical production can be improved using the solvent-tolerant Pseudomonas putida S12 in a two-liquid-phase system consisting of aqueous media and a water-immiscible octanol phase with production of 3-methylcatechol from toluene as the model conversion. For this purpose the genes involved in this conversion, todC1C2BAD from P. putida F1, were introduced into P. putida S12 with high stable expression. Production of 3-methylcatechol was monitored in batch incubations with different media using a single medium and a two-liquid medium–octanol system. The maximum concentration of 3-methylcatechol increased two-fold using the two-liquid medium–octanol system, irrespective of the selected medium.

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Authors and Affiliations

  1. Division of Industrial Microbiology, Department of Food Technology and Nutritional Sciences, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, The Netherlands e-mail: Jan.Wery@imb.ftns.wau.nl Tel.: +31-317-484412 Fax: +31-317-484978, , , , , , NL

    J. Wery & J. A. M. de Bont

  2. UCTA, Campus de Gambelas, Universidade do Algarve, 8000 Faro, Portugal, , , , , , PT

    D. I. Mendes da Silva

Authors
  1. J. Wery
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  2. D. I. Mendes da Silva
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  3. J. A. M. de Bont
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Received: 29 December 1999 / Received revision: 29 February 2000 / Accepted: 6 March 2000

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Wery, J., Mendes da Silva, D. & de Bont, J. A genetically modified solvent-tolerant bacterium for optimized production of a toxic fine chemical. Appl Microbiol Biotechnol 54, 180–185 (2000). https://doi.org/10.1007/s002530000381

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  • DOI: https://doi.org/10.1007/s002530000381

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