Summary
Po66, a mouse IgG1 monoclonal antibody, was produced by immunization against a patient lung squamous cell carcinoma. The tissue reactivity of the antibody was measured by a radioimmunological assay with enzymatically dissociated cells, by an immunofluorescence test on frozen tissue sections and by peroxidase-staining of paraffin sections. The antibody bound to lung squamous cell carcinoma, oesophagous carcinoma and, inconsistantly to lung adenocarcinoma but not to the other tumours tested. Some normal tissues also reacted positively, in particular bronchial serous glands, oesophagus epithelium and renal distal and collecting tubules. In normal and malignant tissues showing epithelioid differentiation, Po66 bound to the intermediate maturation area. The antigen immunoprecipitated by Po66 from lung squamous cell carcinoma appeared as a single band with a molecular weight 47000 to 50000 daltons. Purified monoclonal antibody Po66 and an unrelated IgG1 immunoglobulin were labelled with radioactive iodine and injected i. v. into nude mice bearing subcutaneous xenografts of human lung squamous cell carcinoma. The localization index in the tumour was 3.3. Antibody labelled with 131I allowed gamma-scintigraphic imaging of the xenografts which were clearly outlined by days 9 to 11.
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References
Bourel D, Fauchet R, Chevrinais AM et al. (1982) Anti-HLA A2 and A 28 monoclonal antibody — production and study of the cross reaction. Immunol Lett 5:273
Bourne AJ (1981) Handbook of immunoperoxidase staining methods. DAKO corporation
Brenner BG, Jothy S, Shuster J, Fuks A (1982) Monoclonal antibodies to human lung tumor antigens demonstrated by immunofluorescence and immunoprecipitation. Cancer Res 42:3187
Cole SPC, Campling BG, Louwman IH et al. (1984) A strategy for the production of human antibodies reactive with lung tumor cell lines. Cancer Res 44:2750
Edwards PAW (1985) Heterogeneous expression of cell-surface antigens in normal epithelia and their tumours, revealed by monoclonal antibodies. Br J Cancer 51:149
Galfre G, Milstein C (1981) Preparation of monoclonal antibodies: strategies and procedures. Methods Enzymol 73:3
Harlozinska A, Richter R, Albert Z et al. (1983) Antigenic heterogeneity of human lung cancers. J Natl Cancer Inst 70:427
Harwood PJ, Pedley RB, Boder J et al. (1985) Prolonged localization of a monoclonal antibody against CEA in a human colon tumor xenograft. Br J Cancer 52:797
Hirohashi S, Shimosato Y, Ino Y (1982) Antibodies from EB-virus transformed lymphocytes of lymph nodes adjoining lung cancer. Br J Cancer 46:802
Hsu S, Raine L, Fanger H (1981) The use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase technique. J Histochem 29:577
Hubbard AL, Cohn ZA (1975) Externally disposed plasma membrane proteins: I. Enzymatic iodination of mouse L cells. J Cell Biol 64:438
Kasai M, Saxton RE, Holmes EC et al. (1981) Hybridoma monoclonal antibody use in defined surface antigens on human carcinoma cells. Transplant Proc 13:1942
Kyoizumi S, Akiyama M, Kouno N et al. (1985) Monoclonal antibodies to human squamous cell carcinoma of the lung and their application to tumor diagnosis. Cancer Res 45:3274
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680
Luscher B, Main HY, McDonald HR, Bron C (1984) The mouse Lyt2/3 antigen complex: I mode of association of the subunits with the membrane. Mol Immunol 21:329
Mazauric T, Mitchell KF, Letchworth GJ et al. (1982) Monoclonal antibody-defined human lung cell surface protein antigen. Cancer Res 42:150
Mulshine SL, Cuttita F, Bibro M et al. (1983) Monoclonal antibody that distinguishes non small cell from small cell lung cancer. J Immunol 131:497
Munro-Neville A, Gusterson BA (1985) Monoclonal antibodies and human tumors. Pathological and clinical aspects. Eur J Clin Oncol 21:355
Primus FJ, Deland FH, Goldenberg DM (1984) Monoclonal antibodies for radioimmunodetection of cancer. In: Wright GL (ed) monoclonal antibodies and cancer. Marcel Dekker, New York, Basel, p 305
Saccavini JC, Bradwell AR, Fairweather WS, Dykes PW et al. (1985) Limiting factors in the localization of tumours with radiolabelled antibodies. Immunol Today 6:163
Said JW, Nash G, Bank-Schegel S et al. (1983) Keratin in human lung tumors. Ann J Pathol 113:27
Sikora K, Alderson T, Ellis J et al. (1983) Human hybridoma from patients with malignant disease. Br J Cancer 47:135
Stacker SA, Thompson C, Riglar C, McKenzie IFC (1985) A new breast carcinoma antigen defined by a monoclonal antibody. J Natl Cancer Inst 75:801
Wagenaar SJ, Hilgers J, Schmitz du moulin et al. (1984) Patterns of expression of some new antigens of human bronchial carcinomas. Protides Biol Fluids 31:521
Wright GL (1984) Application of monoclonal antibodies to the studies of human cancer. In: Wright GL (ed) monoclonal antibodies and cancer. Marcel Dekker Inc, New York, Basel, p 1–30
Yarbro JW, Bornstein RS, Mastrangelo MJ (1983) Non small cell carcinoma of the lung. Semin Oncol 10:122
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This work was supported by Association pour la Recherche sur le Cancer
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Dazord, L., Bourel, D., Martin, A. et al. A monoclonal antibody (Po66) directed against human lung squamous cell carcinoma immunolocalization of tumour xenografts in nude mice. Cancer Immunol Immunother 24, 263–268 (1987). https://doi.org/10.1007/BF00205641
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DOI: https://doi.org/10.1007/BF00205641