Summary
Human renal cell cancer (RCC) cell lines, ACHN and KRC/Y, with or without exposure to cytokines, were examined for their susceptibility to lymphokine-activated killer (LAK) cells. Flow-cytometric analysis demonstrated constitutional expression of class I antigen on both cell lines, which was enhanced by interferon α (IFNα), IFNγ and tumor necrosis factor α (TNFα). A 4-h51Cr-release cytotoxicity assay demonstrated that pretreatment of both cell lines with IFNγ or IFNα, but not with TNFα, decreased their susceptibility to LAK cells. IFNγ also decreased susceptibility to natural killer cells in a 16-h51Cr-release cytotoxicity assay. IFNγ treatment decreased the susceptibility of ACHN cells in a dose-dependent manner. “Cold”-target competition assay clearly showed that IFNγ- but not TNFα-pretreated cells compete less effectively than do untreated target cells. Pretreatment with IFNγ, however, increased expression of intercellular adhesion molecule-1 (ICAM-1) to a degree comparable to that with TNFα. Northern blot analyses using a 520-base-pair ICAM-1 cDNA as a probe demonstrated that more 3.3-kb mRNA is expressed in IFNγ- and TNFα-pretreated cells. These results suggest that IFNγ-treated RCC cell lines may reduce their ability to be recognized by LAK cells, and that IFN-induced protection of RCC cell lines against LAK cells may depend upon a mechanism independent of the expression of class I antigens or ICAM-1 on tumor cells.
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Tomita, Y., Watanabe, H., Kobayashi, H. et al. Interferon γ but not tumor necrosis factor α decreases susceptibility of human renal cell cancer cell lines to lymphokine-activated killer cells. Cancer Immunol Immunother 35, 381–387 (1992). https://doi.org/10.1007/BF01789016
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DOI: https://doi.org/10.1007/BF01789016