Zusammenfassung
Aktivitäten der Ektoenzyme 5′-Nukleotidase (5′-N) und Phosphatase wurden gemessen an intakten Zellen aus 15 verschiedenen etablierten Linien menschlicher B- und T-Lymphoblasten. Während Phosphatase auf allen Zellinien exprimiert wird, waren 10 der untersuchten Linien negativ für 5′-N. 5′-N-negative Linien finden sich sowohl unter B- als auch T-Zellen; sie tragen auch keine kryptische Enzymaktivität. In einer 5′-N-positiven Linie folgt die Enzymaktivität der Wachstumskurve mit einem Maximum während der logarithmischen Phase. Andererseits ändert Hemmung der 5′-N nichts an der Wachstumscharakteristik dieser Zellinie. Neuraminidasebehandlung der Zelloberfläche bringt eine Steigerung der Phosphatase-, nicht jedoch der 5′-N-Aktivität mit sich. 5′-N auf zwei B-Zellinien und auf menschlichen peripheren Blutlymphozyten zeigt komplette Kreuzreaktivität mit einem Antiserum gegen 5′-N aus menschlicher Plazenta. Dagegen kreuzreagiert 5′-N einer weiteren Lymphomlinie mit B-Zell-Eigenschaften (EHR-A-Ramos) nicht mit diesem Serum. Die Brauchbarkeit dieser Lymphoblastenlinien als Modellsysteme zum Studium von Immundefizienzen wird diskutiert.
Summary
Activities of the ecto-enzymes 5′-nucleotidase (5′-N) and phosphatase were determined on the surface of intact cells from 15 different established lines of human B- and T-lymphoblasts. Whereas all the lines express phosphatase, 10 of the lines were negative for 5′-N. 5′-N-negative cell lines are found among B as well as T cells, and they do not carry cryptic enzyme activity. In a 5′-N-positive line activity of this enzyme is correlated with growth showing a peak during the logarithmic phase. On the other hand, inhibition of 5′-N does not change the growth curve of this line. Neuraminidase treatment of the cell surface brings about an increase in phosphatase but not in 5′-N activity. 5′-N of two B-cell lines and of human peripheral blood lymphocytes shows complete crossreactivity with an antiserum obtained against human placental 5′-N. However, the enzyme of one lymphoma line with B-cell properties (EHR-A-Ramos) does not cross-react with this serum. The results are discussed with respect to suitability of these lymphoblast lines as model systems for the study of immunodeficiencies.
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Parts of these results have been presented at the “3rd International Symposium on Purine Metabolism in Man” (Madrid, June 11–15, 1979) and the “16.Tagung der Gesellschaft für Anthropologie und Humangenetik“ (Heidelberg, September 26–29, 1979)
With a financial support of the Deutsche Forschungsgemeinschaft (grants SFB 37, Gu 123 and Gu 153)
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Gutensohn, W., Gürtler, L.G., Siegert, W. et al. Ectoenzymes on the surface of cells from human lymphoblastoid lines: 5′-Nucleotidase and phosphatase. Blut 41, 411–420 (1980). https://doi.org/10.1007/BF01007765
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DOI: https://doi.org/10.1007/BF01007765