Summary
In meiotic cells of Saccharomyces cerevisiae, reduction in molecular weights of DNA in alkaline sucrose gradients is observed concomittantly with premeiotic DNA replication and with commitment to recombination. Following the completion of the latter processes, higher molecular weights are obtained. These single-stranded breaks are found in both old and newly synthesised strands. Similar scissions in DNA are also found in a temperature-sensitive mutant (cdc40/cdc40), which does not undergo commitment to recombination at the restrictive temperature, and in vegetative wild type cells that were previously exposed to sporulation medium. The suggestion that these scissions are the physical manifestation of commitment to recombination is therefore rejected.
Similar content being viewed by others
References
Bachrach, U., Friedmann, A.: Appl. Microbiol. 22, 706–715 (1971)
Esposito, R. E., Esposito, M. S.: Proc. Nat. Acad. Sci. USA 71, 3172–3176 (1974)
Hotta, Y., Stern, H.: Chromosoma 46, 279–291 (1974)
Jacobson, G. K., Piffon, R., Esposito, R. E., Esposito, M. S.: Proc. Nat. Acad. Sci. USA 72, 1887–1891 (1975)
Johnston, L. H., Williamson, D. H.: Mol. Gen. Genet. 164, 217–225 (1978)
Kassir, Y., Simchen, G.: Genetics 90, 49–68 (1978)
Levine, D., Hutchinson, F.: J. Mol. Biol. 75, 495–502 (1973)
Petes, T. D., Fangman, W. L.: Proc. Nat. Acad. Sci. USA 69, 1188–1191 (1972)
Piñon, R., Salts, Y.: Proc. Nat. Acad. Sci. USA 74, 2850–2854 (1977)
Piñon, R., Salts, Y., Simchen, G.: Exp. Cell Res. 83, 231–238 (1974)
Salts, Y., Simchen, G., Piñon, R.; Mol. Gen. Genet. 146, 55–59 (1976)
Sherman, F., Roman, H.: Genetics 48, 255–261 (1963)
Silva-Lopez, E., Zamb, T. J., Roth, R.: Nature 253, 212–214 (1975)
Simchen, G., Idar, D., Kassir, Y.: Mol. Gen. Genet. 144, 21–27 (1976)
Simchen, G., Piñon, R., Salts, Y.: Exp. Cell Res. 75, 207–218 (1972)
Stern, H., Hotta, Y.: Annu, Rev. Plant Physiol. 29, 415–436 (1978)
Williamson, D. H.: J. Cell Biol. 25, 517–528 (1965)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kassir, Y., Simchen, G. Single-strand scissions of nuclear yeast DNA occur without meiotic recombination. Curr Genet 2, 79–85 (1980). https://doi.org/10.1007/BF00445698
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00445698