Abstract
When the β1-, β2- and β3-tubulin-specific DNAs fromDrosophila melanogaster were used as probes to recognize tubulin-specific sequences in the chromosomes ofDrosophila auraria, they were found to hybridize to the same polytene band in region 32C of the 2L polytene chromosome. Three overlapping clones were isolated from a λEMBL 3 genomic library ofD. auraria, and they all contain β-tubulin-specific sequences based on hybridization and partial-sequencing experiments of subcloned fragments. These clones hybridize in situ to the same polytene chromosome band in region 32C and they represent an approximately 35-kb fragment of genomic DNA.
References
Benton WD, Davis RW (1977) Science 196:180–182
Biolojan S, Falkenburg D, Renkawitz-Pohl R (1984) EMBO J 3:2543–2548
Cleveland DW, Sullivan KF (1985) Annu Rev Biochem 54:331–365
Feinberg AP, Vogelstein B (1983) Anal Biochem 132:6–13
Gasch A, Hinz U, Leiss D, Renkawitz-Pohl R (1988) Mol Gen Gen 211:8–16
Gasch A, Hinz U, Renkawitz-Pohl R (1989) Proc Natl Acad Sci USA 86:3215–3218
Kastritsis CD, Scouras ZG, Ashburner M (1986) Chromosoma 93:381–385
Leiss D, Hinz U, Gasch A, Mertz R, Renkawitz-Pohl R (1988) Development 104:525–531
Leumeunier F, David JR, Tsacas L, Ashburner M (1986) Themelanogaster species group. In: Ashburner M, Carson HL, Thompson J (eds) The Genetics and Biology of Drosophila, vol 3e. Academic Press, London, Orlando, pp 148–156
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Michiels F, Falkenburg D, Muller AM, Hinz U, Otto U, Bellmann R, Glatzer K-H, Brand R, Biolojan S, Renkawitz-Pohl R (1987) Chromosoma 95:387–395
Michiels F, Gasch A, Kaltschmidt B, Renkawitz-Pohl R (1989) EMBO J 8:1559–1565
Natzle JE, McCarthy BJ (1984) Dev Biol 104:187–198
Purdue ML (1986) In: Roberts DB (ed)Drosophila a practical approach. IRL Press, Oxford, Washington DC, p 111
Renkawitz-Pohl R, Glatzer KH, Kunz W (1980) Nucleic Acids Res 8:4593–4611
Rudolph JE, Kimble M, Hoyle HD, Subler MA, Raff EC (1987) Mol Cell Biol 7:2231–2242
Sanchez F, Natzle JE, Clevel DW, Kirschner MW, McCarthy BJ (1980) Cell 22:845–854
Scouras ZG, Kastritsis CD (1984) Chromosoma 89:96–106
Author information
Authors and Affiliations
Additional information
Communicated by K. Wolf
Rights and permissions
About this article
Cite this article
Scouras, Z.G., Milioni, D., Yiangou, M. et al. Theβ−tubulin genes ofDrosophila auraria are arranged in a cluster. Curr Genet 25, 84–87 (1994). https://doi.org/10.1007/BF00712973
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00712973