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Ginkgolide B production in cultured cells derived from Ginkgo biloba L. leaves

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Abstract

Callus cultures and cell suspension cultures derived from Ginkgo biloba L. leaves produced ginkgolidc B. In cell suspension cultures, the production reached a maximum by the 13th day of subculture and followed by a sharp decrease. The medium of Murashige and Skoog induced the highest ginkgolide B content in cultures while the medium of Schenk and Hildebrandt promoted cell growth. For the maximal production of ginkgolide B, cells were cultured in Murashige and Skoog medium modified to contain 1.0 mg/l of α-naphthaleneacetic acid, 0.1 mg/1 of kinetin, 30 g/1 sucrose and 1.25 mM potassium phosphate with a molar ratio of ammonium to nitrate ions of 1 ∶ 3.

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Abbreviations

B5:

Gamborg et al (1968) medium

GKB:

Ginkgolide B

MS:

Murashige and Skoog (1962) medium

NAA:

α-naphthaleneacetic aicd

SH:

Schenk and Hildebrandt (1972) medium

References

  • van Beek TA, Scheeren HA, Rantio T, Melger WC and Lelyveld GP (1991) J. Chromatogr. 543: 375–387

    Google Scholar 

  • Braquet P (1987) Drugs of the Future 12: 643–646

    Google Scholar 

  • Carrier DJ, Cosentino G, Neufeld R, Rho D, Weber M, Archambault J (1990) Plant Cell Rep. 8: 635–638

    Google Scholar 

  • Corey EJ, Kang MC, Desai MC, Ghosh AK, Houpis IN (1988) J. Am. Chem. Soc. 110: 649–651

    Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Exp. Cell Res. 50: 151–158.

    Google Scholar 

  • Huh H, Staba EJ (1993) Plante Med. 59: 232–239

    Google Scholar 

  • Jeon MH, Sung SH, Jeon S, Huh H, Kim J, Kim YC (1993) Arch. Pharm. Res. 16: 244–250

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol. Plant 15: 473–497

    Google Scholar 

  • Nakanishi K (1967) Pure Appl. Chem. 14: 89–113

    Google Scholar 

  • Nakanishi K, Habaguchi K (1971) J. Am. Chem. Soc. 93: 3546–3547

    Google Scholar 

  • Schenk RV, Hildebrandt AC (1972) Can. J. Bot. 50: 199–204

    Google Scholar 

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Authors and Affiliations

  1. College of Pharmacy, Seoul National University, San 56-1 Shillim-Dong, 151-742, Kwanak-Gu, Seoul, Korea

    Mee Hee Jeon, Sang Hyun Sung, Hoon Huh & Young Choong Kim

Authors
  1. Mee Hee Jeon
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  2. Sang Hyun Sung
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  3. Hoon Huh
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  4. Young Choong Kim
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Communicated by F. Constabel

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Jeon, M.H., Sung, S.H., Huh, H. et al. Ginkgolide B production in cultured cells derived from Ginkgo biloba L. leaves. Plant Cell Reports 14, 501–504 (1995). https://doi.org/10.1007/BF00232783

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  • DOI: https://doi.org/10.1007/BF00232783

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